TY - JOUR TI - Intracellular Staining for Phosphorylated STAT4 and STAT5 in Mouse Splenocytes AU - Villegas-Mendez, Ana AU - de Souza, J. Brian AU - Lavelle, Seen-Wai AU - Findlay, Emily Gwyer AU - Shaw, Tovah N. AU - Saris, Christiaan J. AU - Hunter, Christopher A. AU - Riley , Eleanor M. AU - Couper, Kevin N. VL - 4 IS - 3 PY - 2014 DA - 2014/02/05 SP - e1043 C1 - Bio-protocol 2014;4:e1043 DO - 10.21769/BioProtoc.1043 UR - https://doi.org/10.21769/BioProtoc.1043 AB - The Stat (Signal Transducer and Activator of Transcription) family of proteins are critical signal transducers involved in fundamental cellular processes, including cell growth and differentiation, development, apoptosis, immune responses and inflammation. In here, we describe a simple and reproducible flow cytometry protocol to measure Stat protein phosphorylation in splenocyte preparations from malaria infected mice. JF - Bio-protocol SN - 2331-8325 PB - Bio-protocol LLC. BIO101 - False