Request a Protocol - Bio-protocol

Request a Protocol

As part of our efforts to make science more reproducible, we're working with Bio-protocol to link the protocols they host with research published at Science Advances.

To request a protocol for Science Advances paper that's not yet available on Bio-protocol, simply fill in the form below and they will send a request to the author. If the author chooses to make it available, the Bio-protocol team will let you know via email and a link will be added to the Science Advances article. Your details will not be shared with the authors unless you opt-in to share them below.

Research Article

ATAC-seq identifies thousands of extrachromosomal circular DNA in cancer and cell lines
Pankaj Kumar et al.

DOI: 10.1126/sciadv.aba2489

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ATAC-seq library preparation

Identification of eccDNA from ATAC-seq and WGS libraries

Copy number amplification analysis

eccDNA isolation

Outward-directed PCRs (inverse PCR) for detection of eccDNA

Metaphase FISH

List of TCGA IDs that were used for LGG and GBM data analysis

Testing the limit of detection of gene amplification by CNV measurements

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