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Research Article

ATAC-seq identifies thousands of extrachromosomal circular DNA in cancer and cell lines
Pankaj Kumar et al.

DOI: 10.1126/sciadv.aba2489

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ATAC-seq library preparation View detailed protocol

Identification of eccDNA from ATAC-seq and WGS libraries View detailed protocol

Copy number amplification analysis

eccDNA isolation View detailed protocol

Outward-directed PCRs (inverse PCR) for detection of eccDNA View detailed protocol

Metaphase FISH View detailed protocol

List of TCGA IDs that were used for LGG and GBM data analysis

Testing the limit of detection of gene amplification by CNV measurements

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