Cell Biology

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    Protocols in Current Issue
    Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
    Authors:  Tanvir Hossain, Shahla Romal and Tokameh Mahmoudi, date: 04/20/2022, view: 750, Q&A: 0
    [Abstract]

    The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected in the absence of a curative therapy until recently. One of the limitations for in vitro HBV research has been the absence of high titer and pure recombinant HBV

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    Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
    Authors:  Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren and Oliver M. Sieber, date: 04/20/2022, view: 1255, Q&A: 0
    [Abstract]

    Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success,

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    Generation, Maintenance and HBV Infection of Human Liver Organoids
    Authors:  Shahla Romal, Tanvir Hossain and Tokameh Mahmoudi, date: 03/20/2022, view: 1314, Q&A: 0
    [Abstract]

    Hepatitis B virus (HBV) infection represents a major public health problem infecting approximately 400 million people worldwide. Despite the availability of a preventive vaccine and anti-viral therapies, chronic HBV infection remains a major health issue because it increases the risk of developing liver cirrhosis and hepatocellular carcinoma

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    Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection
    [Abstract]

    The ubiquitous and cancer-associated Epstein-Barr virus (EBV) is associated with nearly all cases of nasopharyngeal carcinoma (NPC). Nasopharyngeal tissue is comprised of both pseudostratified and stratified epithelium, which are modeled in three-dimensional (3-D) cell culture. The cellular origin of EBV-associated NPC is as yet unknown, but both

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    Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability
    Authors:  Luisa Klemke, Joshua P. Blume, Tiago De Oliveira and Ramona Schulz-Heddergott, date: 01/20/2022, view: 2399, Q&A: 0
    [Abstract]

    Organoids are complex three-dimensional structures, which contain different cell types and help to overcome many limitations of conventional 2D cell culture techniques. Here, we present a protocol for the cultivation of murine matched-pairs of small intestinal and colonic epithelial organoids, and colonic tumor organoids derived from the chemical

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    From 3D to 2D: Harmonization of Protocols for Two-dimensional Cultures on Cell Culture Inserts of Intestinal Organoids from Various Species
    [Abstract]

    In the expanding field of intestinal organoid research, various protocols for three- and two-dimensional organoid-derived cell cultures exist. Two-dimensional organoid-derived monolayers are used to overcome some limitations of three-dimensional organoid cultures. They are increasingly used also in infection research, to study physiological

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    OrganoPlate Micro-fluidic Microvessel Culture and Analysis
    Authors:  Abidemi Junaid and Thomas Hankemeier, date: 07/05/2021, view: 2642, Q&A: 0
    [Abstract]

    The endothelial cells from the microvasculature are key drivers and targets of inflammatory and thrombotic processes in microvascular diseases. The study of bioactive lipids in inflammatory processes has been largely based on two-dimensional endothelial cell cultures. Three-dimensional microvessels-on-a-chip provides an opportunity to monitor the

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    Generation and Maintenance of Homogeneous Human Midbrain Organoids
    Authors:  Henrik Renner, Martha Grabos, Hans R. Schöler and Jan M. Bruder, date: 06/05/2021, view: 3313, Q&A: 0
    [Abstract]

    Three-dimensional cell cultures (“organoids”) promise to better recapitulate native tissue physiology than traditional 2D cultures and are becoming increasingly interesting for disease modeling and compound screening efforts. While a number of protocols for the generation of neural organoids have been published, most protocols require

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    A Robust Mammary Organoid System to Model Lactation and Involution-like Processes
    Authors:  Elsa Charifou, Jakub Sumbal, Zuzana Koledova, Han Li and Aurélie Chiche, date: 04/20/2021, view: 4194, Q&A: 0
    [Abstract]

    The mammary gland is a highly dynamic tissue that changes throughout reproductive life, including growth during puberty and repetitive cycles of pregnancy and involution. Mammary gland tumors represent the most common cancer diagnosed in women worldwide. Studying the regulatory mechanisms of mammary gland development is essential for understanding

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    A Method to Efficiently Cryopreserve Mammalian Cells on Paper Platforms
    Authors:  Muhammedin Deliorman, Pavithra Sukumar, Roaa Alnemari and Mohammad A. Qasaimeh, date: 09/20/2020, view: 2962, Q&A: 0
    [Abstract] This protocol describes a simple method to cryopreserve mammalian cells within filter papers as an alternative to conventional slow-freezing approach. The method involves treating paper fibers with fibronectin, using low concentrations of the cryoprotectant dimethyl sulfoxide (DMSO), and slow freezing cells to -80 °C at a 1 °C min-1 ...



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