Biophysics

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    Protocols in Current Issue
    Purification of Mitochondrial Ribosomal Complexes from Trypanosoma cruzi and Leishmania tarentolae for Cryo-EM Analysis
    Authors:  Stéphanie Durrieu-Gaillard, Marie Sissler and Yaser Hashem, date: 05/20/2022, view: 919, Q&A: 1
    [Abstract]

    Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis, caused by Trypanosoma cruzi, Trypanosoma brucei, and various Leishmania spp., respectively. They harbor a single large mitochondrion that is essential for the survival of the parasite. Interestingly,

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    Quantitative Analysis of Actin Cable Length in Yeast
    Authors:  Shane G. McInally, Jane Kondev and Bruce L. Goode, date: 05/05/2022, view: 936, Q&A: 0
    [Abstract]

    Polarized actin cables in S. cerevisiae are linear bundles of crosslinked actin filaments that are assembled by two formins, Bnr1 (localized to the bud neck), and Bni1 (localized to the bud tip). Actin is polymerized at these two sites, which results in cables extending along the cell cortex toward the back of the mother cell. These cables serve

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    Bsoft: Image Processing for Structural Biology
    Author:  J. Bernard Heymann, date: 04/20/2022, view: 1194, Q&A: 0
    [Abstract]

    Bsoft is a software package primarily developed for processing electron micrographs, with the goal of determining the structures of biologically relevant molecules, molecular assemblies, and parts of cells. However, it incorporates many ways to deal with images, from the mundane to very sophisticated algorithms. This article is an introduction

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    Single Molecule Tracking Nanoscopy Extended to Two Colors with MTT2col for the Analysis of Cell-Cell Interactions in Leukemia
    Authors:  Loriane Maillot, Magali Irla and Arnauld Sergé, date: 04/20/2022, view: 806, Q&A: 0
    [Abstract]

    Single molecule tracking (SMT) is a powerful technique to study molecular dynamics, and is particularly adapted to monitor the motion and interactions of cell membrane components. Assessing interactions among two molecular populations is classically performed by several approaches, including dual-color videomicroscopy, which allows monitoring of

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    Quantitative and Anatomical Imaging of Human Skin by Noninvasive Photoacoustic Dermoscopy
    Authors:  Zhiyang Wang, Fei Yang, Wuyu Zhang and Sihua Yang, date: 04/05/2022, view: 506, Q&A: 0
    [Abstract]

    Imaging plays a vital role in the diagnosis and treatment of skin diseases. However, pure optical imaging technique is limited to the visualization of superficial skin tissues. Ultrasonic imaging technique can detect deep tissues, but it lacks detailed information on microscopic pathological structures. Photoacoustic imaging is an advanced

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    Tracking Moving Cells in 3D Time Lapse Images Using 3DeeCellTracker
    Authors:  Chentao Wen and Koutarou D. Kimura, date: 02/20/2022, view: 1401, Q&A: 0
    [Abstract]

    Recent advancements in 3D microscopy have enabled scientists to monitor signals of multiple cells in various animals/organs. However, segmenting and tracking the moving cells in three-dimensional time-lapse images (3D + T images), to extract their dynamic positions and activities, remains a considerable bottleneck in the field. We developed a deep

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    Femtoliter Injection of ESCRT-III Proteins into Adhered Giant Unilamellar Vesicles
    [Abstract]

    The endosomal sorting complex required for transport (ESCRT) machinery mediates membrane fission reactions that exhibit a different topology from that observed in clathrin-coated vesicles. In all of the ESCRT-mediated events, the nascent vesicle buds away from the cytosol. However, ESCRT proteins are able to act upon membranes with different

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    NBD-lipid Uptake Assay for Mammalian Cell Lines
    Authors:  Sara Abad Herrera, Marta Grifell-Junyent and Thomas Günther Pomorski, date: 02/20/2022, view: 1716, Q&A: 0
    [Abstract]

    All eukaryotic cells are equipped with transmembrane lipid transporters, which are key players in membrane lipid asymmetry, vesicular trafficking, and membrane fusion. The link between mutations in these transporters and disease in humans highlights their essential role in cell homeostasis. Yet, many key features of their activities, their

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    Single Molecular Resolution to Monitor DNA Replication Fork Dynamics upon Stress by DNA Fiber Assay
    Author:  Wenpeng Liu, date: 12/20/2021, view: 1386, Q&A: 0
    [Abstract]

    DNA replication always encounters numerous endogenous and exogenous stresses during the cell cycle. Measuring the cell responses to stress has primarily relied on cell survival and incorporation of radioactive dNTPs, which is limited in resolution. A higher resolution is required to monitor how replication and repair respond to these stresses.

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    Delivery of AAV for Expression of Fluorescent Biosensors in Juvenile Mouse Hippocampus
    [Abstract]

    Genetically encoded fluorescent biosensors are versatile tools for studying brain metabolism and function in live tissue. The genetic information for these biosensors can be delivered into the brain by stereotaxic injection of engineered adeno-associated viruses (AAVs), which can selectively target different cell types depending on the capsid

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