Microbiology

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    Protocols in Current Issue
    Bacterial Growth Curve Measurements with a Multimode Microplate Reader
    Authors:  Ariel T. Rogers, Kaitlin R. Bullard, Akash C. Dod and Yong Wang, date: 05/05/2022, view: 1009, Q&A: 0
    [Abstract]

    Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution, higher efficiency, and are less laborious, while analysis and interpretation of the microplate-based

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    Cytopathic Effect Assay and Plaque Assay to Evaluate in vitro Activity of Antiviral Compounds Against Human Coronaviruses 229E, OC43, and NL63
    Authors:  Yanmei Hu, Chunlong Ma and Jun Wang, date: 02/05/2022, view: 1829, Q&A: 0
    [Abstract]

    Coronaviruses are important human pathogens, among which the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent for the COVID-19 pandemic. To combat the SARS-CoV-2 pandemic, there is a pressing need for antivirals, especially broad-spectrum antivirals that are active against all seven human coronaviruses (HCoVs).

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    A Phenotypic Screen for the Liver Stages of Plasmodium vivax
    [Abstract]

    Control of malaria caused by Plasmodium vivax can be improved by the discovery and development of novel drugs against the parasite’s liver stage, which includes relapse-causing hypnozoites. Several recent reports describe breakthroughs in the culture of the P. vivax liver stage in 384-well microtiter plates, with the goal of enabling a

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    Sex-specific Separation of Plasmodium falciparum Gametocyte Populations
    Authors:  Melanie C. Ridgway, Daniela Cihalova and Alexander G. Maier, date: 06/05/2021, view: 2099, Q&A: 0
    [Abstract]

    Plasmodium falciparum is a unicellular eukaryotic parasite that causes malaria in humans. The parasite is spread by Anopheles mosquitoes after ingestion of sexual stage parasites known as gametocytes. Malaria transmission depends on parasites switching from the disease-causing asexual blood forms to male and female gametocytes. The current

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    ODELAM: Rapid Sequence-independent Detection of Drug Resistance in Mycobacterium tuberculosis Isolates
    Authors:  Thurston Herricks, Magdalena Donczew, David R. Sherman and John D. Aitchison, date: 05/20/2021, view: 2536, Q&A: 0
    [Abstract]

    Antimicrobial-resistant Mycobacterium tuberculosis (Mtb) causes over 200,000 deaths globally each year. Current assays of antimicrobial resistance require knowledge of the mutations that confer drug resistance or long periods of culture time to test growth under drug pressure. We present ODELAM (One-cell Doubling Evaluation of Living Arrays of

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    Screening for Lysogen Activity in Therapeutically Relevant Bacteriophages
    Authors:  Fernando L. Gordillo Altamirano and Jeremy J. Barr, date: 04/20/2021, view: 5296, Q&A: 1
    [Abstract]

    Lysogenic phages can integrate into their bacterial host’s genome, potentially transferring any genetic information they possess including virulence or resistance genes, and are therefore routinely excluded from therapeutic applications. Lysogenic behavior is typically seen in phages that create turbid plaques or possess subpar bactericidal

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    Primer ID Next-Generation Sequencing for the Analysis of a Broad Spectrum Antiviral Induced Transition Mutations and Errors Rates in a Coronavirus Genome
    [Abstract]

    Next generations sequencing (NGS) has become an important tool in biomedical research. The Primer ID approach combined with the MiSeq platform overcomes the limitation of PCR errors and reveals the true sampling depth of population sequencing, making it an ideal tool to study mutagenic effects of potential broad-spectrum antivirals on RNA viruses.

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    Determination of Antibacterial Activity of Film Coatings against Four Clinically Relevant Bacterial Strains
    [Abstract]

    Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium,

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    Evaluation of Toxicity with Brine Shrimp Assay
    Authors:  Christina N. Banti and Sotiris K. Hadjikakou, date: 01/20/2021, view: 3788, Q&A: 0
    [Abstract]

    The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with

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    Antimicrobial Sensitivity Assay for Bdellovibrio bacteriovorus
    Authors:  Emanuele Marine and Klaas M. Pos, date: 12/20/2020, view: 2169, Q&A: 0
    [Abstract]

    Bdellovibrio bacteriovorus, an obligate predatory bacterium [i.e., bacteria that kill and feed on other bacteria (prey)], has the potential to be used as a probiotic for the disinfection of surfaces or for the treatment of bacterial infections. One option is to use this organism in combination with antimicrobials to potentiate the effectiveness of

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