Medicine

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    Protocols in Current Issue
    A Highly Sensitive Method to Efficiently Profile the Histone Modifications of FFPE Samples
    Authors:  Linxuan Zhao, Vamsi Krishna Polavarapu, Ram Prakash Yadav, Pengwei Xing and Xingqi Chen, date: 05/20/2022, view: 1020, Q&A: 0
    [Abstract]

    The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically important to understand gene regulation in human disease. The required input for current genome-wide

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    Immunomagnetic Isolation and Enrichment of Microvascular Endothelial Cells from Human Adipose Tissue
    [Abstract]

    Human adipose tissue-resident microvascular endothelial cells are not only garnering attention for their emergent role in the pathogenesis of obesity-related metabolic disorders, but are also of considerable interest for vascular tissue engineering due, in part, to the abundant, accessible, and uniquely dispensable nature of the tissue. Here, we

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    Preparation, Characterization, and Cell Uptake of PLGA/PLA-PEG-FA Nanoparticles
    Authors:  Heng Yang, Junsik Sung, Zahra Alghoul and Didier Merlin, date: 04/05/2022, view: 601, Q&A: 0
    [Abstract]

    Oral administration of colon-targeting nanoformulations holds many advantages over the systemic delivery of free drugs, or traditional nontargeting formulations in the treatment of ulcerative colitis (UC). Currently, the most conventional method for constructing colon-targeting drug delivery systems (DDS) is by integrating the biocompatible

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    Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-based Test on HEK-293T hACE2 Cells
    [Abstract]

    Neutralizing antibodies (NAbs) are of particular importance because they can prevent binding of the receptor binding domain (RBD) of the spike protein (S protein) to the angiotensin-converting enzyme 2 (ACE2) receptor present at the surface of human cells, preventing virus entry into the host cells. The gold standard method for detection of

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    Ex-vivo Skin Permeability Tests of Nanoparticles for Microscopy Imaging
    Authors:  Nethanel Friedman, Sharon Merims, Jhonatan Elia and Ofra Benny, date: 04/05/2022, view: 582, Q&A: 0
    [Abstract]

    Delivery of drugs through the skin is a major challenge in the field of drug delivery systems. Quantification of materials, and specifically nanoparticles, within the skin layers is essential for the development of advanced topical and transdermal delivery systems. We have developed a technique for ex-vivo segmentation and evaluation of human

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    HiSAT: A Novel Method for the Rapid Diagnosis of Allergy
    Authors:  Hirotomo Shibaguchi and Yuki Yasutaka, date: 02/05/2022, view: 1080, Q&A: 0
    [Abstract]

    To identify causative substances for allergies to drugs or foods, the lymphocyte transformation test (LTT) is currently widely used as in vitro test, but its accuracy is not satisfactory. We have developed a novel method designated high-sensitivity allergy test (HiSAT) for determining allergy expression by measuring cell kinetics, using the

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    Chronic Daily House Dust Mite Exposure in Mice is an Effective Model to Quantify the Effect of Pharmacologic Agents on Discrete Stages of Artery Remodeling in Pulmonary Hypertension
    Authors:  Lea C. Steffes and Maya E. Kumar, date: 01/05/2022, view: 1654, Q&A: 0
    [Abstract]

    Pulmonary hypertension (PH) is a heterogenous and incurable disease marked by varying degrees of pulmonary vascular remodeling. This vascular remodeling, which includes thickening of the smooth muscle layer (an early finding) and formation of occlusive neointimal lesions (a late finding) in the pulmonary arteries, is a major driver of morbidity

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    Measurement of Reactive Oxygen and Nitrogen Species in Living Cells Using the Probe 2',7'-Dichlorodihydrofluorescein
    Authors:  Waleska Dornas and Detlef Schuppan, date: 12/20/2021, view: 1354, Q&A: 0
    [Abstract]

    Reactive oxygen species and reactive nitrogen species (RONS) are involved in programmed cell death in the context of numerous degenerative and chronic diseases. In particular, the ability of cells to maintain redox homeostasis is necessary for an adaptive cellular response to adverse conditions that can cause damage to proteins and DNA, resulting

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    A Genetically Engineered Mouse Model of Venous Anomaly and Retinal Angioma-like Vascular Malformation
    Authors:  Xudong Cao, Beibei Xu, Xiao Li, Taotao Li and Yulong He, date: 08/05/2021, view: 1911, Q&A: 0
    [Abstract]

    Characterization of key regulators in vein development will advance our understanding of mechanisms underlying venous anomalies and provide therapeutic targets for the treatment of vascular malformations. Here, we provide a detailed protocol for the generation of genetically engineered mouse models targeting the Tek gene for the analysis of vein

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    A Swimming-based Assay to Determine the Exercise Capacity of Adult Zebrafish Cardiomyopathy Models
    Authors:  Xiao Ma and Xiaolei Xu, date: 08/05/2021, view: 1444, Q&A: 0
    [Abstract]

    Exercise capacity, measured by treadmill in humans and other mammals, is an important diagnostic and prognostic index for patients with cardiomyopathy and heart failure. The adult zebrafish is increasingly used as a vertebrate model to study human cardiomyopathy due to its conserved cardiovascular physiology, convenience for genetic manipulation,

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