Glutathione S-transferase-fused DNMT1 was co-transfected into A549 cells with either sh-NC or sh-HDAC1, and 50 μL acetyltransferase assay buffer was incubated in 20 μM acetyl-CoA at 30 °C for 2 h. The reaction mixture was analyzed by SDS-PAGE and further examined through Western blot analysis.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.