BSR-T7 cells were plated into a 96-well plate at 2 × 104 cells per well and transfected the next day with plasmids coding for Flag/L[Renilla] and a P variant with jetPRIME (Polyplus). Luciferase signals were measured 24 hours later according to the manufacturer’s protocol (Renilla Luciferase Assay System, Promega).

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