Molecular Biology


    High-throughput Assessment of Mitochondrial Protein Synthesis in Mammalian Cells Using Mito-FUNCAT FACS
    Authors:  Hironori Saito, Tatsuya Osaki, Yoshiho Ikeuchi and Shintaro Iwasaki, date: 02/05/2023, view: 212, Q&A: 0

    In addition to cytosolic protein synthesis, mitochondria also utilize another translation system that is tailored for mRNAs encoded in the mitochondrial genome. The importance of mitochondrial protein synthesis has been exemplified by the diverse

    Preparation of Caenorhabditis elegans for Scoring of Muscle-derived Exophers
    Authors:  Katarzyna Banasiak, Michał Turek and Wojciech Pokrzywa, date: 01/05/2023, view: 380, Q&A: 0

    Utilizingresources available from the mother's body to guarantee healthy offspring growth is the fundamental reproductive strategy. Recently, we showed that a class of the largest extracellular vesicles known as exophers, which are responsible

    BONCAT-based Profiling of Nascent Small and Alternative Open Reading Frame-encoded Proteins
    Authors:  Xiongwen Cao, Yanran Chen, Alexandra Khitun and Sarah A. Slavoff, date: 01/05/2023, view: 363, Q&A: 0

    RIBO-seq and proteogenomics have revealed that mammalian genomes harbor thousands of unannotated small and alternative open reading frames (smORFs, <100 amino acids, and alt-ORFs, >100 amino acids, respectively). Several dozen mammalian

    Fluorescence Screens for Identifying Central Nervous System–Acting Drug–Biosensor Pairs for Subcellular and Supracellular Pharmacokinetics

    Subcellular pharmacokinetic measurements have informed the study of central nervous system (CNS)–acting drug mechanisms. Recent investigations have been enhanced by the use of genetically encoded fluorescent biosensors for drugs of interest at the

    Proteome Integral Solubility Alteration (PISA) Assay in Mammalian Cells for Deep, High-Confidence, and High-Throughput Target Deconvolution

    Chemical proteomics focuses on the drug–target–phenotype relationship for target deconvolution and elucidation of the mechanism of action—key and bottleneck in drug development and repurposing. Majorly due to the limits of using

    Fluorescent Binding Protein Sensors for Detection and Quantification of Biochemicals, Metabolites, and Natural Products
    Authors:  Salete M. Newton and Phillip E. Klebba, date: 11/20/2022, view: 1010, Q&A: 0

    Membrane transporters and soluble binding proteins recognize particular nutrients, metabolites, vitamins, or ligands. By modifying genetically engineered single cysteine residues near the active sites of such proteins with extrinsic maleimide

    Single-molecule Force Spectroscopy on Biomembrane Force Probe to Characterize Force-dependent Bond Lifetimes of Receptor–ligand Interactions on Living Cells
    Authors:  Tongtong Zhang, Chenyi An, Wei Hu and Wei Chen, date: 10/20/2022, view: 977, Q&A: 0

    The transmembrane receptor–ligand interactions play a vital role in the physiological and pathological processes of living cells, such as immune cell activation, neural synapse formation, or viral invasion into host cells. Mounting evidence

    A Novel Imaging Technique for The On-site Assessment of Renal Biopsy Specimens
    Authors:  Tomoaki Takata, Hajime Isomoto, Takuji Iyama and Kentaro Yamada, date: 09/20/2022, view: 566, Q&A: 0

    When performing renal biopsy, it is necessary to identify the cortex, where glomeruli are exclusively distributed, to ensure the quality of the specimen for histological diagnosis. However, conventional methods using a stereomicroscope or magnifying

    In-Cell Western Protocol for Semi-High-Throughput Screening of Single Clones
    Authors:  Arpita S. Pal, Alejandra M. Agredo and Andrea L. Kasinski, date: 08/20/2022, view: 1618, Q&A: 0

    The in-cell western (ICW) is an immunocytochemical technique that has been used to screen for effects of siRNAs, drugs, and small molecule inhibitors. The reduced time and number of cells required to follow this protocol illustrates its


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