We are pleased to invite submissions for a Special Issue on coronavirus/COVID-19 research. Learn more
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Current Issue
Recognizing the need for greater reproducibility in scientific research, our collaborating journals encourage their
authors to publish step-by-step methods in Bio-protocol/Bio-101.
Why contribute to and use Bio-101?
Contributing to
Bio-101 is
easy and free!

To save authors' time, protocols can be submitted in any format and our dedicated editing team will format the protocols for the authors. Contributions are free of charge.
formatting and
user-driven feedback

Each protocol undergoes proofreading by our editorial team to ensure both quality and clarity. Following this, users can provide feedback and suggestions using our commenting system, enabling post-release updates.
Something missing?
Submit a request!

Users can request new protocols to be written using our "Protocol Request" system, which will then be fielded by our team of Associate Editors or volunteers from the userbase.
New: Expression and Purification of Functionally Active Serotonin 5-HT2A Receptor in Insect Cells Using Low-titer Viral Stock http://bio-protocol.org/e3704 This #protocol can be used to selectively over-express forms of the receptor by varying the post-infection harvest times.
Twitter • 2 days ago
New: Measuring Intracellular Vesicle Density and Dispersion Using Fluorescence Microscopy and ImageJ/FIJI http://bio-protocol.org/e3703 Tracking fluorescent vesicles based on their position relative to cell nuclei allows quantifying and analyzing their distribution. @ufmg @J_Cell_Sci
Twitter • 3 days ago
New: SMART (Single-Molecule Analysis of Resection Tracks) Technique for Assessing DNA end-Resection in Response to DNA Damage http://bio-protocol.org/e3701 A #method to visualize the 3′ single-strand DNA tails formed by treatments with S-phase specific DNA-damaging agents. @IstTumori
Twitter • 3 days ago
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