Plant Science

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    Generating Reproducing Anoxia Conditions for Plant Phenotyping
    Authors:  Iny E. Mathew, Hormat Shadgou Rhein, Ardawna J. Green and Kendal D. Hirschi, date: 02/05/2023, view: 57, Q&A: 0

    Based on the availability of oxygen, plant growth environment can be normoxic (normal environment), hypoxic (reduced oxygen, <21%), or anoxic (complete depletion of oxygen). Hypoxic/anoxic environment is created when a plant is exposed to

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    Assay for Phytaspase-mediated Peptide Precursor Cleavage Using Synthetic Oligopeptide Substrates
    Authors:  Sven Reichardt, Annick Stintzi and Andreas Schaller, date: 02/05/2023, view: 36, Q&A: 0

    Proteases control plant growth and development by limited proteolysis of regulatory proteins at highly specific sites. This includes the processing of peptide hormone precursors to release the bioactive peptides as signaling molecules. The proteases

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    Evaluating Plant Drought Resistance with a Raspberry Pi and Time-lapse Photography
    Authors:  Daniel N. Ginzburg and Seung Y. Rhee, date: 01/20/2023, view: 707, Q&A: 0

    Identifying genetic variations or treatments that confer greater resistance to drought is paramount to ensuring sustainable crop productivity. Accurate and reproducible measurement of drought stress symptoms can be achieved via automated,

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    Targeting Ultrastructural Events at the Graft Interface of Arabidopsis thaliana by A Correlative Light Electron Microscopy Approach

    Combining two different plants together through grafting is one of the oldest horticultural techniques. In order to survive, both partners must communicate via the formation of de novo connections between the scion and the rootstock. Despite the

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    Profiling of Single-cell-type-specific MicroRNAs in Arabidopsis Roots by Immunoprecipitation of Root Cell-layer-specific GFP-AGO1
    Authors:  Lusheng Fan, Bin Gao and Xuemei Chen, date: 12/20/2022, view: 328, Q&A: 0

    MicroRNAs (miRNA) are small (21–24 nt) non-coding RNAs involved in many biological processes in both plants and animals. The biogenesis of plant miRNAs starts with the transcription of MIRNA (MIR) genes by RNA polymerase II; then, the

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    Faster Bacterial Gene Cloning Using the Brick into the Gateway (BiG) Protocol

    Cloning systems like Gateway and Golden Gate/Braid are known because of their efficiency and accuracy. While the main drawback of Gateway is the expensive cost of the enzymes used in its two-step (LR and BP) reaction, Golden Gate requires

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    Measurement of Transgenes Copy Number in Wheat Plants Using Droplet Digital PCR
    Authors:  Peng Liu, Shuang Liu, Jiajia Lei, Jianping Chen and Jian Yang, date: 12/05/2022, view: 351, Q&A: 0

    Genetic transformation is a powerful method for the investigation of gene function and improvement of crop plants. The transgenes copy number in the transgenic line is involved in gene expression level and phenotypes. Additionally, identification of

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    Focused Ion Beam Milling and Cryo-electron Tomography Methods to Study the Structure of the Primary Cell Wall in Allium cepa
    Authors:  William J. Nicolas, Grant J. Jensen and Elliot M. Meyerowitz, date: 12/05/2022, view: 490, Q&A: 0

    Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample

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    X-ray Crystallography: Seeding Technique with Cytochrome P450 Reductase
    Authors:  Bixia Zhang, Jacob A. Lewis, Rishi Hazra and ChulHee Kang, date: 11/05/2022, view: 588, Q&A: 0

    Cytochrome P450 reductase (CPR) is a multi-domain protein that acts as a redox partner of cytochrome P450s. The CPR contains a flavin adenine dinucleotide (FAD)–binding domain, a flavin mononucleotide (FMN)-binding domain, and a connecting

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    Measurement of Ascorbate Peroxidase Activity in Sorghum
    Author:  Praveen Kumar, date: 10/20/2022, view: 900, Q&A: 1

    The ascorbate peroxidase (APX) is a widely distributed antioxidant enzyme. It differs from catalase and other peroxidases in that it scavenges/reduces reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) to water using reduced ascorbate as

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