Developmental Biology

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    GeneWeld: Efficient Targeted Integration Directed by Short Homology in Zebrafish

    Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Multiple design strategies for zebrafish gene targeting have previously been reported with widely varying frequencies for

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    An Improved Method for Individual Tracking of Voluntary Wheel Running in Pair-housed Juvenile Mice
    Authors:  David A. Valientes, Anthony M. Raus and Autumn S. lvy, date: 07/05/2021, view: 616, Q&A: 0

    Rodent cages equipped with access to a voluntary running wheel are commonly used to study the effects of aerobic physical activity on physiology and behavior. Notable discoveries in exercise neurobiology, including the key role of brain-derived

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    A More Standardized Method for Imaging and Measuring Drosophila Thorax Length Using ImageJ
    Authors:  Emily K. Fowler, Stewart Leigh, Amanda Bretman and Tracey Chapman, date: 06/20/2021, view: 841, Q&A: 0

    Thorax measurements are important metrics of Drosophila fitness and are often correlated with key life-history traits, such as lifespan and fecundity. Therefore, thorax length is a widely used measure in studies of genetic variation and plasticity

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    Analysis of Caenorhabditis elegans Sperm Number, Size, Activation, and Mitochondrial Content
    Authors:  Amy M. Hammerquist, Chia-An Yen and Sean P. Curran, date: 06/05/2021, view: 1140, Q&A: 0

    Infertility is a widespread and often unexplained issue. Studying reproduction using C. elegans males offers insight into the influence of individual factors on male fertility in humans. We have created a collection of protocols to assess several

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    Rice Root Hair Phenotypes Imaged by Cryo-SEM

    Cryo-scanning electron microscopy (cryo-SEM) was first introduced for scientific use in the 1980s. Since then, cryo-SEM has become a routine technique for studying the surfaces and internal structures of biological samples with a high water content.

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    Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands
    Authors:  Cheng-I J. Ma and Julie A. Brill, date: 06/05/2021, view: 1116, Q&A: 0

    Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in

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    Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium
    Authors:  Leonie Witte, Karen Linnemannstöns, Mona Honemann-Capito and Julia Christina Gross, date: 06/05/2021, view: 1156, Q&A: 0

    Secretory Wnt trafficking can be studied in the polarized epithelial monolayer of Drosophila wing imaginal discs (WID). In this tissue, Wg (Drosophila Wnt-I) is presented on the apical surface of its source cells before being internalized into the

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    Generation of Mouse Pluripotent Stem Cell-derived Trunk-like Structures: An in vitro Model of Post-implantation Embryogenesis

    Post-implantation mammalian embryogenesis involves profound molecular, cellular, and morphogenetic changes. The study of these highly dynamic processes is complicated by the limited accessibility of in utero development. In recent years, several

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    Isolation of First-Trimester and Full-term Human Placental Hofbauer Cells
    Authors:  Anna Appios, Jake R. Thomas and Naomi McGovern, date: 06/05/2021, view: 1470, Q&A: 0

    The placenta is the crucial organ that regulates the health of both mother and fetus during pregnancy. The human placenta is composed of villous tree-like structures that embed into the maternal decidua. Within the stroma of the villi resides a

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    Intact in situ Preparation of Drosophila melanogaster Lymph Gland for Comprehensive Analysis of Larval Hematopoiesis
    Authors:  Diana Rodrigues, K. VijayRaghavan, Lucas Waltzer and Maneesha S. Inamdar, date: 05/20/2021, view: 2405, Q&A: 0

    Blood cells have a limited lifespan and are replenished by a small number of hematopoietic stem and progenitor cells (HSPCs). Adult vertebrate hematopoiesis occurs in the bone marrow, liver and spleen making a comprehensive analysis of the entire

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