Microbiology

Extracellular RNA Isolation from Biofilm Matrix of Pseudomonas aeruginosa

Authors: Anastasiya E. Scherbakova, Valentina S. Rykova, Kseniya V. Danilova, Andrey I. Solovyev and Daria A. Egorova, date: 11/05/2020, view: 195, Q&A: 0

Most bacteria in natural ecosystems form biofilms-a bacterial community, surrounded by a polymer matrix that consists mostly of exopolysaccharides, proteins, and nucleic acids. Extracellular RNA as a matrix component is involved in biofilm

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Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein

Authors: Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 1422, Q&A: 0

This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...

Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein

Authors: Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 86, Q&A: 0

This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...

Different Methods of Soil DNA Extraction

Authors: Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 3484, Q&A: 0

Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...

One Plate-double Nutrient Endospore Activation Method

Authors: Bhagwan Narayan Rekadwad, Juan M. Gonzalez and Chandrahasya N. Khobragade, date: 01/05/2020, view: 1094, Q&A: 0

This paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing ...

Microfluidic-based Growth and Imaging of Bacterial Biofilms

Authors: James M Brown, Birte Blunk, Paul Williams and Kim R Hardie, date: 12/20/2019, view: 1388, Q&A: 0

Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these ...

Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil

Authors: Laynet A. Puentes , Yusibeska L. Ramos, Ysvic A. Inojosa, César E. Rivera and Angela De Sisto, date: 06/20/2019, view: 1715, Q&A: 0

For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...

Very Low Molecular Weight Proteins Electrophoresis Protocol

Authors: César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 7475, Q&A: 4

The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...

Ty1 Retrotransposition Frequency Assay Using a Chromosomal Ty1his3AI or Ty1kanMXAI Element

Author: M. Joan Curcio, date: 09/05/2018, view: 2793, Q&A: 0

Here I describe a simple genetic assay to determine the frequency of retrotransposition of a single chromosomal Ty1 element that is marked with the retrotransposition indicator gene, his3AI or kanMXAI. The assay is used to ...

Assays for Oxidative Responses of Fusarium graminearum Strains to Superoxide Radicals

Authors: Yan-Zhang Wang, Yan Guo and Wei-Hua Tang, date: 09/05/2018, view: 2786, Q&A: 0

The ascomycete fungus Fusarium graminearum is a major causal agent of Fusarium head blight (FHB), a devastating disease affecting small grains cereals worldwide. To better understand the pathogenesis of this fungus, we provide here ...

Chiasma Counting in Maize Male Meiocytes

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