Microbiology

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    Sclerotinia sclerotiorum Protoplast Preparation and Transformation
    Authors:  Chi Lan, Lulu Qiao and Dongdong Niu, date: 01/05/2023, view: 246, Q&A: 1

    Sclerotinia sclerotiorum causes white mold, leading to substantial losses on a wide variety of hosts around the world. Many genes encoding effector proteins play important roles in the pathogenesis of S. sclerotiorum. Therefore, establishment of a

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    PERK Pathway Inhibitors Cure Group A Streptococcal Necrotizing Fasciitis in a Murine Model

    Group A streptococcus (GAS) is a Gram-positive human pathogen that causes invasive infections with mild to life-threatening severity, like toxic shock syndrome, rheumatic heart disease, and necrotizing fasciitis (NF). NF is characterized by a

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    Stimulation of Human Periodontal Ligament Fibroblasts Using Purified Dentilisin Extracted from Treponema denticola
    Authors:  Sean Ganther, J. Christopher Fenno and Yvonne L. Kapila, date: 12/20/2022, view: 189, Q&A: 0

    Periodontal disease is a chronic multifactorial disease triggered by a complex of bacterial species. These interact with host tissues to cause the release of a broad array of pro-inflammatory cytokines, chemokines, and tissue remodelers, such

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    Infection of the Developing Central Nervous System of Drosophila by Mammalian Eukaryotic and Prokaryotic Pathogens
    Authors:  Billel Benmimoun, Bente Winkler and Pauline Spéder, date: 12/05/2022, view: 287, Q&A: 0

    Pathogen invasion of the central nervous system (CNS) is an important cause of infection-related mortality worldwide and can lead to severe neurological sequelae. To gain access to the CNS cells, pathogens have to overcome the blood–brain

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    Hypochlorite Stress Assay for Phenotypic Analysis of the Halophilic Archaeon Haloferax volcanii Using an Improved Incubation Method and Growth Monitoring
    Authors:  Paula Mondragon, Sungmin Hwang, Amy Schmid and Julie A. Maupin-Furlow, date: 11/20/2022, view: 438, Q&A: 0

    The study of haloarchaea provides an opportunity to expand understanding of the mechanisms used by extremophiles to thrive in and respond to harsh environments, including hypersaline and oxidative stress conditions. A common strategy used to

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    Efficient Generation of Genome-wide Libraries for Protein–ligand Screens Using Gibson Assembly
    Authors:  Tamara Sternlieb, Mira Loock, Mengjin Gao and Igor Cestari, date: 11/20/2022, view: 731, Q&A: 0

    Genome-wide screens using yeast or phage displays are powerful tools for identifying protein–ligand interactions, including drug or vaccine targets, ligand receptors, or protein–protein interactions. However, assembling libraries

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    Fluorescent Binding Protein Sensors for Detection and Quantification of Biochemicals, Metabolites, and Natural Products
    Authors:  Salete M. Newton and Phillip E. Klebba, date: 11/20/2022, view: 972, Q&A: 0

    Membrane transporters and soluble binding proteins recognize particular nutrients, metabolites, vitamins, or ligands. By modifying genetically engineered single cysteine residues near the active sites of such proteins with extrinsic maleimide

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    Babesia duncani in Culture and in Mouse (ICIM) Model for the Advancement of Babesia Biology, Pathogenesis and Therapy
    Authors:  Vandana Kumari, Anasuya C. Pal, Pallavi Singh and Choukri Ben Mamoun, date: 11/20/2022, view: 383, Q&A: 0

    Babesiosis is a tick-borne disease caused by pathogens belonging to the genus Babesia. In humans, the disease presents as a malaria-like illness and can be fatal in immunocompromised and elderly people. In the past few years, human babesiosis has

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    In vitro Di-ubiquitin Formation Assay and E3 Cooperation Assay
    Authors:  Rebecca J. Burge, Katie H. Jameson, Anthony J. Wilkinson and Jeremy C. Mottram, date: 11/05/2022, view: 592, Q&A: 0

    Ubiquitination is a post-translational modification conserved across eukaryotic species. It contributes to a variety of regulatory pathways, including proteasomal degradation, DNA repair, and cellular differentiation. The ubiquitination of substrate

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    Negative Staining Transmission Electron Microscopy of HIV Viral Particles Permeabilized with PFO and Capsid Stabilized with IP6
    Authors:  Derrick Lau, Chantal L. Márquez, Michael W. Parker and Till Böcking, date: 10/20/2022, view: 688, Q&A: 0

    The human immunodeficiency virus 1 (HIV-1) consists of a viral membrane surrounding the conical capsid. The capsid is a protein container assembled from approximately 1,500 copies of the viral capsid protein (CA), functioning as a reaction and

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