Continuous Measurement of Reactive Oxygen Species Formation in Bacteria-infected Bone Marrow–derived Macrophages Using a Fluorescence Plate Reader

    Macrophages are at the center of innate immunity and are the main target cells of the intracellular pathogen Salmonella enterica serovar Typhi. The production of reactive oxygen and nitrogen species (ROS/RNS) is the host’s early response to

    Sample Preparation and Integrative Data Analysis of a Droplet-based Single-Cell ATAC-sequencing Using Murine Thymic Epithelial Cells

    Accessible chromatin regions modulate gene expression by acting as cis-regulatory elements. Understanding the epigenetic landscape by mapping accessible regions of DNA is therefore imperative to decipher mechanisms of gene regulation under

    Safety Profiling of Tumor-targeted T Cell–Bispecific Antibodies with Alveolus Lung- and Colon-on-Chip

    Traditional drug safety assessments often fail to predict complications in humans, especially when the drug targets the immune system. Rodent-based preclinical animal models are often ill-suited for predicting immunotherapy-mediated adverse

    Modelling Graft-Versus-Host Disease in Mice Using Human Peripheral Blood Mononuclear Cells

    Graft-versus-host disease (GvHD) is a significant complication of allogeneic hematopoietic stem cell transplantation. In order to develop new therapeutic approaches, there is a need to recapitulate GvHD effects in pre-clinical, in vivo systems, such

    Improved Macrophage Enrichment from Mouse Skeletal Muscle

    Macrophages are a heterogeneous class of innate immune cells that offer a primary line of defense to the body by phagocytizing pathogens, digesting them, and presenting the antigens to T and B cells to initiate adaptive immunity. Through specialized

    Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification
    Authors:  Serena Sensi and Andreas Goebel, date: 12/05/2022, view: 260, Q&A: 0

    Immunoglobulins are proteins produced by the immune system, which bind specifically to the antigen that induced their formation and target it for destruction. Highly purified human immunoglobulins are commonly used in research laboratories for

    Murine Double Hit Model for Neonatal Cardiopulmonary Diseases: Bronchopulmonary Dysplasia (BPD) and Pulmonary Hypertension Associated with BPD
    Authors:  Steven P. Garrick, Philip J. Berger, Marcel F. Nold and Claudia A. Nold-Petry, date: 11/05/2022, view: 554, Q&A: 0

    Bronchopulmonary dysplasia (BPD) and pulmonary hypertension associated with BPD (BPD-PH) are of multifactorial origin and share common risk factors. Most murine models of BPD expose newborn pups to only one of these risk factors—more commonly

    Flow Cytometry Analysis of SIRT6 Expression in Peritoneal Macrophages

    The sirtuin 6 has emerged as a regulator of acute and chronic immune responses. Recent findings show that SIRT6 is necessary for mounting an active inflammatory response in macrophages. In vitro studies revealed that SIRT6 is stabilized in the

    High-Efficiency Retroviral Transduction for Type 1 Regulatory T Cell Differentiation
    Authors:  Michael C. McGee, Avery August and Weishan Huang, date: 09/05/2022, view: 772, Q&A: 0

    Type 1 regulatory T (Tr1) cells are an immunoregulatory CD4+ Foxp3- IL-10high T cell subset with therapeutic potential for various inflammatory diseases. Retroviral (RV) transduction has been a valuable tool in defining the signaling pathways and

    Combination of Sterile Injury and Microbial Contamination to Model Post-surgical Peritoneal Adhesions in Mice
    Authors:  Julia Bayer, Deborah Stroka, Paul Kubes, Daniel Candinas and Joel Zindel, date: 08/20/2022, view: 1290, Q&A: 0

    Abdominal surgeries are frequently associated with the development of post-surgical adhesions. These are irreversible fibrotic scar bands that appear between abdominal organs and the abdominal wall. Patients suffering from adhesions are at risk of


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