• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Cancer Biology

In vitro Human Umbilical Vein Endothelial Cells (HUVEC) Tube-formation Assay Authors:  Josephine MY Ko and Maria Li Lung, date: 09/20/2012, view: 48419, Q&A: 1
Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to their receptors, release of proteases to ...
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In vivo Matrigel Plug Angiogenesis Assay Authors:  Hong Lok Lung and Maria Li Lung, date: 09/20/2012, view: 36183, Q&A: 4
The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce ...
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Neuroscience

Mouse Transient Global Ischemia Two-Vessel Occlusion Model Authors:  Fabrizio Pontarelli, Dimitry Ofengeim, R. Suzanne Zukin and Elizabeth A. Jonas, date: 09/20/2012, view: 12144, Q&A: 0
Transient global ischemia in rodents induces delayed death of hippocampal CA1 neurons, as well as in some hilar neurons of the dentate gyrus, medium aspiny neurons of the striatum, pyramidal neurons in neocortical layers II, V and VI, and Purkinje neurons of the cerebellum. In contrast to focal ischemia that mimics regional stroke in humans, this ...
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Plant Science

Isolation of Rice Embryo Single Cell Type using Laser Capture Microdissection (LCM) Author:  Tie Liu, date: 09/20/2012, view: 8236, Q&A: 0
A lot of transcriptional profiling in plant and animals has used RNAs samples from many different cell types. The laser-capture microdissection (LCM) can identify and harvest pure cellular populations directly from heterogenous tissues based on histological identification. The molecules or protein isolated from LCM-captured cells can be suitable ...
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Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves Authors:  Arsalan Daudi and Jose A. O’Brien, date: 09/20/2012, view: 53126, Q&A: 20
In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous methods (Thordal-Christensen et al., 1997; Bindschedler et al., 2006; Daudi et al. ...
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