• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast Author:  Yuehua Wei, date: 06/20/2012, view: 11483, Q&A: 0
One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible. Hyperhosphorylated proteins usually migrate slowlier than dephosphorylated proteins. However, not all phosphorylated proteins can be readily detected, due to sub-optimal sample ...
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Cancer Biology

Cell Survival Rate Assay Author:  FengZhi Liu, date: 06/20/2012, view: 13601, Q&A: 0
This protocol utilizes PicoGreen 96-well plate technology. This method is applied to estimate the sensitivity of different tumor cell lines to chemodrugs.
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Primary Tumor Preparation Author:  Liang Lei, date: 06/20/2012, view: 11598, Q&A: 0
This protocol has been developed for culturing primary glioblastoma cells. We have most experience in using it on rodent preparations, but it can also be used in culturing cells from other species.
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Immunology

Generation of Mouse Bone Marrow-Derived Macrophages (BM-MFs) Authors:  Ivan Zanoni, Renato Ostuni and Francesca Granucci, date: 06/20/2012, view: 14979, Q&A: 1
Generating mouse macrophages from bone-marrow progenitor cells is a useful tool to study biological functions of mouse macrophages. Macrophages are one of the major populations of phagocytes and play many different roles during inflammatory process initiation and termination.
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Generation of Mouse Bone Marrow-Derived Dendritic Cells (BM-DCs) Authors:  Francesca Granucci, Renato Ostuni and Ivan Zanoni, date: 06/20/2012, view: 16450, Q&A: 0
Generating mouse dendritic cells from bone-marrow progenitor cells is a useful tool to study biological functions of mouse dendritic cells. Dendritic cells are one of the major populations of phagocytes able to activate both innate and adaptive immune cells.
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Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-cardiolipin Antibodies Author:  Zheng Liu, date: 06/20/2012, view: 10331, Q&A: 0
The circulating anticardiolipin antibody is a hallmark of antiphospholipid syndrome. It also appears in a number of autoimmune mouse models and is indicative of the break of tolerance against self antigens. This protocol describes a reliable method to determine the relative serum titer of anticardiolipin in autoimmune mouse models.
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Molecular Biology

A Simple RNA Preparation Procedure from Yeast for Northern Blot Using Hot Phenol Author:  Yuehua Wei, date: 06/20/2012, view: 11980, Q&A: 0
Compared to several expensive RNA extraction kits, the following protocol provides an economic and simple method for researchers to extract yeast RNA. This method can achieve RNA quality that is sufficient for most northern blot studies in yeast.
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