• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Quantification of Chitinase Activity in Fusarium oxysporum Authors:  Carmen Ruiz-Roldan and M. Isabel G. Roncero, date: 08/05/2016, view: 7234, Q&A: 1
Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase activity might be useful to identify structural ...
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Analytical Gel Filtration for Probing Heavy Metal Transfer between Proteins Authors:  Steffen Lorenz Drees and Mathias Lübben, date: 08/05/2016, view: 5862, Q&A: 0
Heavy metals can cause damage to biomolecules such as proteins and DNA in multiple ways. Cells therefore strive for keeping intracellular (heavy) metal ions bound to specific proteins that are capable of handling detoxification, export or integration as cofactors. Metal binding proteins usually provide specific coordination sites that bind certain ...
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Cell Biology

A Live-imaging, Heat Shock-inducible System to Measure Aux/IAA Degradation Rates in Planta Authors:  Jessica M. Guseman, Jennifer L. Nemhauser and Britney L. Moss, date: 08/05/2016, view: 6066, Q&A: 0
An emerging theme in biology is the importance of cellular signaling dynamics. In addition to monitoring changes in absolute abundance of signaling molecules, many signal transduction pathways are sensitive to changes in temporal properties of signaling components (Purvis and Lahav, 2013). The phytohormone auxin regulates myriad processes in plant ...
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Cell Tracer Violet and CellTracker Red CMTPX Staining of Purified Mature Plasmodium-infected Red Blood Cells Authors:  Henrique Borges da Silva, Carlos Eduardo Tadokoro and Maria Regina D’Império Lima, date: 08/05/2016, view: 6026, Q&A: 0
Efficient staining methods to identify Plasmodium-infected red blood cells (iRBCs) are crucial to discriminate precisely the immune cells responsible for their elimination from circulation. Here, we describe the protocol for the purification of iRBCs and their subsequent staining with the vital dyes Cell Tracer Violet (CTV) or CellTracker ...
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Mouse Liver Mitochondria Isolation, Size Fractionation, and Real-time MOMP Measurement Authors:  Thibaud T. Renault, Mark P.A Luna-Vargas and Jerry E. Chipuk, date: 08/05/2016, view: 6499, Q&A: 0
The mitochondrial pathway of apoptosis involves a complex interplay between dozens of proteins and lipids, and is also dependent on the shape and size of mitochondria. The use of cellular models in past studies has not been ideal for investigating how the complex multi-factor interplay regulates the molecular mechanisms of mitochondrial outer ...
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Immunology

In vivo DCs Depletion with Diphtheria Toxin and MARCO+/MOMA1+ Cells Depletion with Clodronate Liposomes in B6.CD11c-DTR Mice Authors:  Henrique Borges da Silva, Carlos Eduardo Tadokoro and Maria Regina D’Império Lima, date: 08/05/2016, view: 5838, Q&A: 0
To evaluate precisely the relative roles of different splenic phagocytic cells during an immune response, efficient methods for the depletion of specific populations are needed. Here, we describe the protocols for the depletion of splenic dendritic cells (DCs) by human diphtheria toxin (DTx) treatment in target mice (which express the human DTx ...
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Microbiology

A Highly Efficient Method for Measuring Oxygen Consumption Rate in Fusarium graminearum Authors:  Daniel Gebhard, Jakob Bönnighausen, Jörg Bergemann, Wilhelm Schäfer and Jörg Bormann, date: 08/05/2016, view: 5871, Q&A: 0
The filamentous ascomycete Fusarium graminearum is the causal agent of Fusarium head blight, a devastating disease of cereals with a worldwide distribution. Fusarium graminearum infections result in a quantitative yield reduction by impairing the growth of the kernels, and a qualitative reduction by poisoning the ...
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Antifungal and Zearalenone Inhibitory Activity of Ocimum sanctum L. Essential Oil on Fusarium graminearum Determined by UHPLC and RT-qPCR Authors:  Naveen Kumar Kalagatur, Nirmaladevi Dhamodaran, Chandranayaka Siddaiah, Venkataramana Mudili and Murali Harishchandra Sreepathi, date: 08/05/2016, view: 5220, Q&A: 0
Fusarium graminearum has been given special attention in the context of agricultural commodities due to its ability to grow in diverse climatic conditions, and to produce different mycotoxins including zearalenone (ZEA) and type-B trichothecenes, which cause ill health effects on humans, animals and plants. The application of synthetic ...
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Molecular Biology

Generation of IgG-Fc Glycovariants Using Recombinant Glycosidases and Glycosyltransferases Authors:  Isaak Quast, Michael A. Maurer and Jan D. Lünemann, date: 08/05/2016, view: 7179, Q&A: 0
The immunoglobulin G (IgG) fragment crystallizable (Fc) domain contains a single, highly conserved asparagine 297 (N297) glycosylation site in the CH2 domain, which is buried within the hydrophobic core of each of the two heavy chains. The biantennary core glycan structure, composed of 2 N-acetylglucosamine (GlcNAc) and 3 mannose residues, can be ...
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Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120 Authors:  Kangming Chen, Huilan Zhu, Liping Gu, Shengni Tian and Ruanbao Zhou, date: 08/05/2016, view: 5686, Q&A: 0
Anabaena sp. strain PCC 7120 has long served as a model organism for investigating N2-fixation, photosynthesis, and various plant-type metabolic pathways and biofuel production, as well as cellular differentiation (Xu et al., 2008, Halfmann et al., 2014, Golden and Yoon, 2003). Since more than 30,000 sequenced ...
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Capillary Electrophoresis in Hydroxyethylcellulose Solutions for the Analysis of dsDNA, dsRNA, and siRNA Authors:  Chenchen Liu, Yoshinori Yamaguchi and Xiaoming Dou, date: 08/05/2016, view: 5107, Q&A: 0
Capillary electrophoresis (CE) is identified as a promising technology for the study of nucleic acid molecules because of its high efficiency, high throughput with automation and integration. Compared to the traditional method of slab gel electrophoresis (SGE), the advantages of CE cannot be emphasized more. Most of CE process, including sample ...
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Neuroscience

Acute Live/Dead Assay for the Analysis of Toxic Effects of Drugs on Cultured Neurons Authors:  Noa Sadeh, Efrat Oni-Biton and Menahem Segal, date: 08/05/2016, view: 7197, Q&A: 0
The primary culture of central nervous system (CNS) neurons is a popular test system for a rapid, quantitative and reliable assessment of the effects of drugs on central neurons. Consequently, studies on the excitotoxicity of NMDA activation and on intracellular calcium handling machineries with respect to ischemic damage to the brain as well as ...
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Plant Science

EST-SSR Analysis and Cross-species Transferability Study in Lavandula Authors:  Ayelign M. Adal, Zerihun A. Demissie and Soheil S. Mahmoud, date: 08/05/2016, view: 5516, Q&A: 0
The genus Lavandula comprises of several economically important lavender species that are mainly cultivated worldwide for essential oil production. Identification of lavender species and their cultivars has been a huge bottleneck in lavender industries due to lack of appropriate identification mechanisms. Recent advances in ...
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Stem Cell

Neurosphere Co-culture Assay Authors:  Fanny Ehret, Steffen Vogler and Gerd Kempermann, date: 08/05/2016, view: 7912, Q&A: 0
The hippocampal niche is one of two areas in the brain where stem cells reside. In this neurogenic niche, stem cells can be found in close proximity to astrocytes and in contact with microvessels consisting of pericytes and endothelial cells. To study the regulatory interplay of this complex niche network in a simplified in vitro model, ...
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Bone Marrow Mesenchymal Stem Cells Adhesion Assay Authors:  Zhigang Yang and Ran Xiao, date: 08/05/2016, view: 6251, Q&A: 0
Mesenchymal stem cells (MSCs) are widespread in adult organisms and involved in tissue maintenance and repair as well as in the regulation of hematopoiesis and immunologic responses. As cell adhesion play important roles in cell interactions and signaling, to thoroughly evaluate the adhesion ability of MSCs is of vital importance to clarify the ...
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