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About Us
For Authors
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Volume 11, 2021
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Biochemistry
RAB21 Activity Assay Using GST-fused APPL1
Authors:
Steve Jean
and
Amy A. Kiger
,
date:
02/20/2016,
view:
6089,
Q&A:
0
The Rab family of small GTPases are essential regulators of membrane trafficking events. As with other small GTPase families, Rab GTPases cycle between an inactive GDP- bound state and an active GTP-bound state. Guanine nucleotide exchange factors (GEFs) promote Rab activation with the exchange of bound GDP for GTP, while GTPase-activating ...
More >>
Cancer Biology
In vitro
Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
Authors:
Shin-Ae Kang
,
Sandra Bajana
and
Takemi Tanaka
,
date:
02/20/2016,
view:
10621,
Q&A:
1
Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood ...
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Cell Biology
VAMP8-3xHA Uptake Assay in HeLa Cells
Authors:
Steve Jean
and
Amy A. Kiger
,
date:
02/20/2016,
view:
5926,
Q&A:
0
Transmembrane proteins are rarely exclusively localized to a specific vesicle or an organelle. Most transmembrane proteins undergo complicated trafficking routes. Thus, transmembrane proteins are under constant flux, and at steady state, found on a variety of vesicles or organelles. This characteristic makes the study of their trafficking routes ...
More >>
Immunology
Isolation of
Nippostrongylus brasiliensis
Larvae from Mouse Lungs
Authors:
Mali Camberis
,
Tiffany Bouchery
and
Graham Le Gros
,
date:
02/20/2016,
view:
8639,
Q&A:
0
The rodent parasite
Nippostrongylus brasiliensis
(
N. brasiliensis
) models the salient features of helminth infection including skin penetration, migration from tissues to lung, maturation and egg production in the gut. As a potent activator of systemic and mucosal Th2 immune responses,
Nippostrongylus brasiliensis
has ...
More >>
Dye Labeling of Live
Nippostrongylus brasiliensis
Larvae for Visualization in Host Tissue
Authors:
Tiffany Bouchery
,
Mali Camberis
and
Graham Le Gros
,
date:
02/20/2016,
view:
6758,
Q&A:
0
Visualization of the interaction between parasitic nematodes and their host enables a better understanding of the development of the nematode during the infectious stages of its life cycle and of the effects of host response on nematode integrity in tissues. Appropriate live imaging of these nematode/host interactions, to date has been hindered by ...
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Neuroscience
Repeated Cross-fostering Protocol as a Mouse Model of Early Environmental Instability
Authors:
Alessandra Luchetti
,
Marco Battaglia
and
Francesca R. D’Amato
,
date:
02/20/2016,
view:
6587,
Q&A:
0
Early life events have a crucial role in programming the individual phenotype indeed the exposure to traumatic experiences during infancy can increase later risks for a variety of neuropsychiatric conditions, including mood and anxiety disorders. Several studies in rodents demonstrated the impact of short and long sessions of separation/isolation ...
More >>
Synaptoneurosome Preparation from C57BL/6 Striata
Authors:
Emma Puighermanal
,
Anne Biever
and
Emmanuel Valjent
,
date:
02/20/2016,
view:
10636,
Q&A:
0
Activity-dependent local mRNA translation endows synapses to remodel their structure and function (Bramham and Wells, 2007). This process is tightly controlled by the state of phosphorylation of several components of the translational machinery including initiation factors and ribosomal proteins (Buffington
et al.
, 2014). The present ...
More >>
Stem Cell
Immunofluorescent Staining of Mouse Intestinal Stem Cells
Authors:
Kevin P. O’Rourke
,
Lukas E Dow
and
Scott W Lowe
,
date:
02/20/2016,
view:
26910,
Q&A:
1
Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, ...
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Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
Authors:
Kevin P. O’Rourke
,
Sarah Ackerman
,
Lukas E Dow
and
Scott W Lowe
,
date:
02/20/2016,
view:
22426,
Q&A:
6
In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild ...
More >>
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