• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Past Issues in 2019

Volume: 9 Issue: 9

Biochemistry

Optimized Oxidative Stress Protocols for Low-microliter Volumes of Mammalian Plasma Author:  Evan A. Langille, date: 05/05/2019, view: 406, Q&A: 0
Small blood volumes commonly obtained from small mammals during field studies are only sufficient for a single biochemical assay. In this study, we used blood collected from a population of wild eastern chipmunks (Tamias striatus) and developed modified methods to improve analytical selectivity and sensitivity required for measuring ...
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Separation of Natural Collagen Crosslinks Using Buffer and Ion-pairing Agent Free Solvents on Silica Hydride Column for Mass Spectrometry Detection Authors:  Rafea Naffa and Joseph Pesek, date: 05/05/2019, view: 332, Q&A: 0
In this protocol we describe the separation of collagen crosslinks in biological tissues and samples including skin, tendon, cartilage, bone and urine. The existing methods use either cation exchange chromatography followed by post-column derivatization with ninhydrin or reverse phase chromatography with mass spectrometry detection. The cation ...
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Cancer Biology

3D Co-culture System of Mouse Prostatic Wild-type Fibroblasts with Human Prostate Cancer Epithelial Cells Authors:  Veronica R. Placencio-Hickok, Manabu Kato and Neil A. Bhowmick, date: 05/05/2019, view: 551, Q&A: 0
Heterogeneous prostatic carcinoma-associated fibroblasts (CAF) contribute to tumor progression. This was established using transgenic mouse models. Paracrine interactions between fibroblasts and epithelial cells were further interrogated using isolated 2D cell culture systems, but 3D culture systems currently being developed can better mimic ...
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Cell Biology

Triple Fluorescence Anisotropy Reporter Imaging in Living Cells Authors:  Brian Ross, Shenq Huey Wong, Nicole E. Snell, Jin Zhang and M. A. Rizzo, date: 05/05/2019, view: 453, Q&A: 0
FRET-based genetically encoded biosensors incorporate two fluorescent proteins into their design to enable ratiometric biosensing of signaling activities in live cells. While emission ratios are generally useful for quantitative studies, they leave little room in the optical spectrum for additional sensors and optogenetic tools. Homotransfer-based ...
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Quantification of Cutaneous Ionocytes in Small Aquatic Organisms Authors:  Garfield T. Kwan, Shane H. Finnerty, Nicholas C. Wegner and Martin Tresguerres, date: 05/05/2019, view: 342, Q&A: 0
Aquatic organisms have specialized cells called ionocytes that regulate the ionic composition, osmolarity, and acid/base status of internal fluids. In small aquatic organisms such as fishes in their early life stages, ionocytes are typically found on the cutaneous surface and their abundance can change to help cope with various metabolic and ...
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Microbiology

Analysis of Indole-3-acetic Acid (IAA) Production in Klebsiella by LC-MS/MS and the Salkowski Method Authors:  Shraddha Gang, Sheetal Sharma, Meenu Saraf, Martin Buck and Jörg Schumacher, date: 05/05/2019, view: 457, Q&A: 0
Many rhizobacteria isolated from plant rhizosphere produce various phytohormones in the form of secondary metabolites, the most common of which is Indole-3-acetic acid (IAA). Here, we detail analytical protocols of IAA detection and quantification, in vitro and in situ, as recently applied to Klebsiella SGM 81, a ...
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Molecular Biology

Improved HTGTS for CRISPR/Cas9 Off-target Detection Authors:  Jianhang Yin, Mengzhu Liu, Yang Liu and Jiazhi Hu, date: 05/05/2019, view: 379, Q&A: 0
Precise genome editing is essential for scientific research and clinical application. At present, linear amplification-mediated high-throughput genome-wide translocation sequencing (LAM-HTGTS) is one of most effective methods to evaluate the off-target activity of CRISPR-Cas9, which is based on chromosomal translocation and employs a “bait” DNA ...
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Neuroscience

Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor Authors:  Elizabeth M. Avegno and Nicholas W. Gilpin, date: 05/05/2019, view: 297, Q&A: 0
Alcohol use disorder (AUD) is a significant public health and economic burden and is often characterized by repeated bouts of alcohol intoxication and withdrawal. Virtually all organ systems are impacted by chronic alcohol exposure. These effects can be investigated using the rat as a model organism; however, rats typically will not ...
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Plant Science

Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling Authors:  Laurent Simon and Feth el Zahar Haichar, date: 05/05/2019, view: 398, Q&A: 0
One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...
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