• Volume 11, 2021
  • Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
Image Credit: BioProtoc.3178


A Radioisotope-free Oligosaccharyltransferase Assay Method Authors:  Takahiro Yamasaki and Daisuke Kohda, date: 03/05/2019, view: 3510, Q&A: 0
Glycosylation of asparagine residues is widespread in Eukarya, and occurs in virtually all Archaea and some eubacterial species. A membrane-bound enzyme, oligosaccharyltransferase, catalyzes the transfer of an oligosaccharide chain from a sugar donor (lipid-linked oligosaccharide, LLO) to an asparagine residue in the consensus sequence, ...
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Cell Biology

Single-cell Motility Analysis of Tethered Human Spermatozoa Authors:  William M. Skinner, Nadja Mannowetz, Polina V. Lishko and Nadia R. Roan, date: 03/05/2019, view: 4821, Q&A: 0
Vigorous sperm flagellar motility is essential for fertilization, and so the quantitative measurement of motility is a useful tool to assess the intrinsic fertility potential of sperm cells and explore how various factors can alter sperm’s ability to reach the egg and penetrate its protective layers. Human sperm beat their flagella many times each ...
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A New Efficient Method for Measuring Oxygen Consumption Rate Directly ex vivo in Human Epidermal Biopsies Authors:  Daniel Schniertshauer, Daniel Gebhard and Jörg Bergemann, date: 03/05/2019, view: 4534, Q&A: 0
Skin cells are constantly exposed to environmental influences such as air pollution, chemicals, pathogens and UV radiation. UV radiation can damage different biological structures, but most importantly cellular DNA. Mitochondria contain their own genome and accumulate UV-induced DNA mutations to a large extent. This can result, e.g., in ...
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Developmental Biology

An Improved Staining Method for Low Signal LacZ Reporter Detection in Mouse Embryos Authors:  Xiaopeng Shen, Feng Xu, Shen Wu, Meng Li, Jingyi Zhang, Rui Liang and Yu Liu, date: 03/05/2019, view: 4314, Q&A: 0
In many fields of biology, especially in the field of developmental biology, LacZ reporter staining is an approach used to monitor gene expression patterns. In the LacZ reporter system, the LacZ gene is inserted in the endogenous location of the target gene via gene knock-in or by constructing a transgenic cassette in ...
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Characterization of Mouse Adult Testicular Macrophage Populations by Immunofluorescence Imaging and Flow Cytometry Authors:  Noushin Mossadegh-Keller and Michael H. Sieweke, date: 03/05/2019, view: 3943, Q&A: 0
Testicular macrophages (tMΦ) are the most abundant immune cells residing in the testis, an immune-privileged organ. TMΦ are known to exhibit different functions, such as protecting spermatozoa from auto-immune attack by producing immunosuppressive cytokines and trophic roles in supporting spermatogenesis and male sex hormone production. They also ...
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Early Life Ovalbumin Sensitization and Aerosol Challenge for the Induction of Allergic Airway Inflammation in a BALB/c Murine Model Authors:  Kyle T Mincham, Naomi M Scott, Jean-Francois Lauzon-Joset, Jonatan Leffler, Philip A Stumbles, Patrick G Holt and Deborah H Strickland, date: 03/05/2019, view: 3993, Q&A: 0
The early life period represents a time of immunological plasticity whereby the functionally immature immune system is highly susceptible to environmental stimulation. Perennial aeroallergen and respiratory viral infection induced sporadic episodes of lung inflammation during this temporal window represent major risk factors for initiation of ...
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Quantification of Serum Ovalbumin-specific Immunoglobulin E Titre via in vivo Passive Cutaneous Anaphylaxis Assay Authors:  Kyle T Mincham, Jonatan Leffler, Naomi M Scott, Jean-Francois Lauzon-Joset, Philip A Stumbles, Patrick G Holt and Deborah Strickland, date: 03/05/2019, view: 3811, Q&A: 0
Murine models of allergic airway disease are frequently used as a tool to elucidate the cellular and molecular mechanisms of tissue-specific asthmatic disease pathogenesis. Paramount to the success of these models is the induction of experimental antigen sensitization, as indicated by the presence of antigen-specific serum immunoglobulin E. The ...
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Molecular Biology

QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs Authors:  Alla Fishman and Ayelet T. Lamm, date: 03/05/2019, view: 5341, Q&A: 0
Small RNAs (sRNAs) are 20-30 nt long non-coding RNA molecules that regulate essentially all cellular processes. Besides being an intensively studied topic in academic research, sRNAs also hold a promise as clinical biomarkers. While the need for expressional profiling of sRNAs is growing, preparation of sRNA libraries for high-throughput ...
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Acute Cerebellar Slice Preparation Using a Tissue Chopper Authors:  Yubin Wang and Michel Baudry, date: 03/05/2019, view: 3688, Q&A: 0
Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
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Plant Science

Detection of Disulfides in Protein Extracts of Arabidopsis thaliana Using Monobromobimane (mBB) Authors:  Shin‐nosuke Hashida and Maki Kawai-Yamada, date: 03/05/2019, view: 4097, Q&A: 0
Thiol-disulfide exchange is a key posttranslational modification, determining the folding process of intra- and inter-protein structures. Thiols can be detected by colorimetric reagents, which are stoichiometrically reduced by free thiols, and by fluorescent adducts, showing fluorescence only after thioester formation. We adapted a simple ...
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Stem Cell

Differentiation of Human Induced Pluripotent Stem Cells (iPSCs) into an Effective Model of Forebrain Neural Progenitor Cells and Mature Neurons Authors:  Scott Bell, Nuwan C. Hettige, Heika Silveira, Huashan Peng, Hanrong Wu, Malvin Jefri, Lilit Antonyan, Ying Zhang, Xin Zhang and Carl Ernst, date: 03/05/2019, view: 10687, Q&A: 0
Induced Pluripotent Stem Cells (iPSCs) are pluripotent stem cells that can be generated from somatic cells, and provide a way to model the development of neural tissues in vitro. One particularly interesting application of iPSCs is the development of neurons analogous to those found in the human forebrain. Forebrain neurons play a central ...
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