Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
Aksiniya Asenova University of California, Davis
12 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos Weill Cornell Medicine
7 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor
Andrea Puhar
  • Faculty, MIMS - Umeå University
Research focus
  • Immunology
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 2 Author merit
Lab Protocol Hub

Education

Ph.D., University of Padova, Italy, 2007

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

2 Protocols published
Dye-uptake Experiment through Connexin Hemichannels
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 7385, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...
Induction of Connexin-hemichannel Opening
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 5257, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...
6 Protocols reviewed
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 5551, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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36 Protocols edited
Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
Authors:  Anthony Yammine, Jinlong Gao and Ann H. Kwan, date: 06/05/2019, view: 290, Q&A: 0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
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Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells
Authors:  Nicholas Mikolajewicz and Svetlana V. Komarova, date: 05/20/2019, view: 586, Q&A: 0
Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous ...
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