Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
Aksiniya Asenova University of California, Davis
12 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos Weill Cornell Medicine
6 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor
Andrea Puhar
  • Faculty, MIMS - Umeå University
Research focus
  • Immunology
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 2 Author merit
Lab Protocol Hub

Education

Ph.D., University of Padova, Italy, 2007

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

2 Protocols published
Dye-uptake Experiment through Connexin Hemichannels
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 7171, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...
Induction of Connexin-hemichannel Opening
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 5100, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...
6 Protocols reviewed
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 5210, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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33 Protocols edited
Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro
Authors:  He Sun, Yuanyuan Luo and Yansong Miao, date: 12/05/2018, view: 812, Q&A: 0
Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin ...
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Extraction and Analysis of Bacterial Teichoic Acids
Authors:  Kelvin Kho and Timothy C. Meredith, date: 11/05/2018, view: 990, Q&A: 0
Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall ...
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