Pankaj Borgohain —BIO-PROTOCOL

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Sanjib Kumar Panda Department of Life Science and Bioinformatics, Assam University, India, India,
1 protocol

Sanjenbam Sanjibia Devi Department of Life Science and Bioinformatics, Assam University, India, India,
1 protocol

Bhaben Chowardhara Department of Life Science and Bioinformatics, Assam University, India, India,
1 protocol

Bedabrata Saha Agricultural Research Organisation
1 protocol

Pankaj Borgohain

Department of Life Science and Bioinformatics, Assam University, India, India,

Research focus

Plant science

1 Author merit
Reviewer merit
Editor merit

Education

M.Sc, Assam University, India

Current position

Research Scholar, Life Science & Bioinformatics, Assam University, India

1 Protocol published

Qualitative Analysis of Lipid Peroxidation in Plants under Multiple Stress Through Schiff’s Reagent: A Histochemical Approach

Authors: Jay Prakash Awasthi, Bedabrata Saha, Bhaben Chowardhara, Sanjenbam Sanjibia Devi, Pankaj Borgohain and Sanjib Kumar Panda, date: 04/20/2018, view: 9839, Q&A: 3

Lipid peroxidation is a physiological indicator of both biotic and abiotic stress responses, hence is often used as a biomarker to assess stress-induced cell damage or death. Here we demonstrate an easy, quick and cheap staining method to assess ...

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TINA AGARWAL

PURDUE UNIVERSITY

Has anyone tried this method with leaf tissue, can the whole leaf be stained or do we need to make section or leaf disc is needed for staining any suggestion?

5/18/2021 11:20:03 AM Reply

TINA AGARWAL

PURDUE UNIVERSITY

Thanks Bedabrata, I did take a look at your paper, so yeah! thank you for sharing that, and will definitely get back to you in case I have any further questions.

2021-05-21 08:14:41

TINA AGARWAL

PURDUE UNIVERSITY

Thank you!

2021-05-21 08:08:37

Bedabrata Saha

Agricultural Research Organisation

Hi Tina,
Thank you for asking. We ourselves have used this protocol for rice leaf tissue segments.
Sahoo, S., Saha, B., Awasthi, J. P., Omisun, T., Borgohain, P., Hussain, S., ... & Panda, S. K. (2019). Physiological introspection into differential drought tolerance in rice cultivars of North East India. Acta Physiologiae Plantarum, 41(4), 1-15.
Besides whole leaf can also be used for staining. It just depends on how you want to represent. If you are facing any problem while performing the experiment feel free to contact us again.

2021-05-19 23:46:55

Jay Awasthi

Department of Life Science and Bioinformatics, Assam University, India, India,

We can take whole leaf or small leaf section for this experiment. Thanks for your questions.

2021-05-19 23:43:28