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Gregory Finnigan
Kansas State University
2 protocols
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Rachael Marie Giersch
Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,
Research focus
Microbiology
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1
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1 Protocol published
Method for Multiplexing CRISPR/Cas9 in
Saccharomyces cerevisiae
Using Artificial Target DNA Sequences
Authors:
Rachael M. Giersch
and
Gregory C. Finnigan
,
date:
09/20/2017,
view:
8548,
Q&A:
0
Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...
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