Rachael Marie Giersch —BIO-PROTOCOL

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Gregory Finnigan Kansas State University
2 protocols

Rachael Marie Giersch

Biochemistry and Molecular Biophysics, Kansas State University, USA, USA,

1 Author merit
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1 Protocol published

Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences

Authors: Rachael M. Giersch and Gregory C. Finnigan, date: 09/20/2017, view: 6390, Q&A: 0

Genome manipulation has become more accessible given the advent of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) editing technology. The Cas9 endonuclease binds a single stranded (single guide) RNA (sgRNA) fragment that ...

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