Melanie Ott Gladstone Institute for Virology and Immunology, The University of California San Francisco, USA
3 protocols
Marielle Cavrois Gladstone Institutes and University of California, San Francisco
7 protocols

Pooja Saxena Medicago R&D Inc.
6 protocols

Vaibhav Shah The University of New South Wales
13 protocols

Vamseedhar Rayaprolu Cell Biology and Neuroscience, Montana State University
22 protocols

Reviewer
Daniela Boehm
  • Gladstone Institute of Virology and Immunology
Research focus
  • Molecular biology
  • 2 Author merit
2 Protocols published
Flow Cytometric Analysis of HIV-1 Transcriptional Activity in Response to shRNA Knockdown in A2 and A72 J-Lat Cell Lines
Authors:  Daniela Boehm and Melanie Ott, date: 06/05/2017, view: 4139, Q&A: 0
The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity ...
Flow Cytometric Analysis of Drug-induced HIV-1 Transcriptional Activity in A2 and A72 J-Lat Cell Lines
Authors:  Daniela Boehm and Melanie Ott, date: 05/20/2017, view: 5336, Q&A: 0
The main obstacle to eradicating HIV-1 from patients is post-integration latency (Finzi et al., 1999). Antiretroviral treatments target only actively replicating virus, while latent infections that have low or no transcriptional activity ...
2 Protocols reviewed
Viral Chromosome Conformation Capture (V3C) Assays for Identifying Trans-interaction Sites between Lytic Viruses and the Cellular Genome
Authors:  Kinjal Majumder, Maria Boftsi and David J Pintel, date: 03/20/2019, view: 341, Q&A: 0
The folding mechanisms of the mammalian genome package our genetic material into the nucleus, and in doing so, dictate its appropriate replication and expression. Chromosome conformation capture technology has enabled the dissection of the folding ...
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Analysis of Early Phase HIV-1 Replication and Integration Events by Using Real-time PCR
Authors:  Graf Laura, Steven P. Moran, Sally Ro, Carlos M.C. de Noronha and Binshan Shi, date: 02/20/2019, view: 512, Q&A: 0
Upon entry into a host cell, the HIV-1 virus undergoes a series of critical early replication events including reverse transcription, nuclear import, and integration of its cDNA into the host genome. Molecular assays used to detect and analyze ...
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