Kae-Jiun Chang —BIO-PROTOCOL

Home About Us For Authors Submit Protocol Archive

Matthew Rasband Program in Developmental Biology and Department of Neuroscience, Baylor College of Medicine, USA
1 protocol

Emmanuelle Berret Ecole Polytechnique Fédérale de Lausanne
9 protocols

Geoffrey Lau City University of Hong Kong
13 protocols

Hong-guang Xia Zhejiang University
7 protocols

Jingli Cao Duke University Medical Center
11 protocols

Reviewer

Kae-Jiun Chang

Post-Doc, University of California, San Francisco

Research focus

Neuroscience

1 Author merit
Reviewer merit
Editor merit

Education

PhD, Baylor College of Medicine

1 Protocol published

Cell Surface Protein-protein Binding on COS-7 Cells

Authors: Kae-Jiun Chang and Matthew N. Rasband, date: 01/05/2014, view: 6995, Q&A: 0

Examination of interactions between a transmembrane protein and a soluble protein by pull-down or immunoprecipitation assays can be tricky and complicated due to the detergent extraction of membrane proteins during the lysate preparation step. The ...

More >

12 Protocols reviewed

Visualizing Filamentous Actin in Chlamydomonas reinhardtii

Authors: Evan W. Craig and Prachee Avasthi, date: 06/20/2019, view: 917, Q&A: 0

This protocol aims to visualize the filamentous actin network in Chlamydomonas reinhardtii. We improved fixed-cell labeling conditions using the F-actin probe, phalloidin. We created a Chlamydomonas-optimized protocol by halving ...

More >

Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons

Authors: Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc, date: 01/05/2018, view: 2686, Q&A: 0

Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of ...

More >

More protocols >