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A peer-reviewed protocol journal. No publication fee; no access fee.
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Matthew Rasband
Program in Developmental Biology and Department of Neuroscience, Baylor College of Medicine, USA
1 protocol
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Emmanuelle Berret
Ecole Polytechnique Fédérale de Lausanne
10 protocols
Geoffrey Lau
City University of Hong Kong
18 protocols
Hong-guang Xia
Zhejiang University
7 protocols
Jingli Cao
Duke University Medical Center
11 protocols
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Reviewer
Kae-Jiun Chang
Post-Doc, University of California, San Francisco
Research focus
Neuroscience
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
PhD, Baylor College of Medicine
1 Protocol published
Cell Surface Protein-protein Binding on COS-7 Cells
Authors:
Kae-Jiun Chang
and
Matthew N. Rasband
,
date:
01/05/2014,
view:
8395,
Q&A:
0
Examination of interactions between a transmembrane protein and a soluble protein by pull-down or immunoprecipitation assays can be tricky and complicated due to the detergent extraction of membrane proteins during the lysate preparation step. The ...
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12 Protocols reviewed
Visualizing Filamentous Actin in
Chlamydomonas reinhardtii
Authors:
Evan W. Craig
and
Prachee Avasthi
,
date:
06/20/2019,
view:
2981,
Q&A:
0
This protocol aims to visualize the filamentous actin network in
Chlamydomonas reinhardtii
. We improved fixed-cell labeling conditions using the F-actin probe, phalloidin. We created a
Chlamydomonas
-optimized protocol by halving ...
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Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
Authors:
Katarina Trajkovic
,
Hyunkyung Jeong
and
Dimitri Krainc
,
date:
01/05/2018,
view:
4513,
Q&A:
0
Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of ...
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