George Diallinas Faculty of Biology, University of Athens, Greece
1 protocol
Ali Kermani University of Michigan, Ann Arbor
8 protocols

Belen Sanz Complutense University
6 protocols

Elizabeth Libby Harvard Medical School
19 protocols

Emily Cope Northern Arizona University
13 protocols

Editor
Emilia Krypotou
  • Post-Doc, Yale University
Research focus
  • Microbiology
  • Molecular Microbiology, Bacterial pathogens, Fungal transporters
  • 1 Author merit

Education

PhD, National and Kapodistrian University of Athens, 2015

Lab information

Eduardo Groisman lab

Publications

1. Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, substrate docking, and mutational analysis identify residues essential for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter. J Biol Chem. 2012, 287: 36792-803.
2. Krypotou E, Diallinas G. Transport assays in filamentous fungi: kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genet Biol. 2014, 63: 1-8. doi: 10.1016/j.fgb.2013.12.004.
3. Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Mol Microbiol. 2014, 93: 129-45. doi: 10.1111/mmi.12646.
4. Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genet Biol. 2015, 75: 56-63. doi: 10.1016/j.fgb.2015.01.009.
5. Krypotou E., Evangelidis T., Bobonis J., Pittis A.A., Gabaldón T., Scazzocchio C., Mikros E., Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Mol Microbiol. 2015, 96: 927-50. doi: 10.1111/mmi.12982.
6. Lougiakis N, Gavriil ES, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G. Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorg Med Chem. 2016, 24: 5941-52.
7. Listeria Placental Infection. Vázquez-Boland JA, Krypotou E, Scortti M.
MBio. 2017 Jun 27;8(3). pii: e00949-17. doi: 10.1128/mBio.00949-17.
8. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Krypotou E, Scortti M, Grundström C, Oelker M, Luisi BF, Sauer-Eriksson AE, Vázquez-Boland J.Cell Rep. 2019 Feb 12;26(7):18151827.e5.ndoi:10.1016/j.celrep.2019.01.073.
1 Protocol published
巢状曲霉转运蛋白动力学分析实验
Authors:  Emilia Krypotou and George Diallinas, date: 11/20/2013, view: 7213, Q&A: 0
Transport assays allow the direct kinetic analysis of a specific transporter by measuring apparent Km and Vmax values, and permit the characterization of substrate specificity profiles through competition ...
11 Protocols reviewed
MNase Digestion for Nucleosome Mapping in Neurospora
Authors:  Cigdem Sancar, Gencer Sancar and Michael Brunner, date: 06/05/2016, view: 7943, Q&A: 0
Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase ...
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XTT Assay of Antifungal Activity
Authors:  Flávio V. Loures and Stuart M. Levitz, date: 08/05/2015, view: 8329, Q&A: 0
XTT assay is a colorimetric method that uses the tetrazolium dye, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) to quantify cell-mediated damage to fungi. Actively respiring fungal cells convert the water-soluble XTT ...
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9 Protocols edited
Cytoduction and Plasmiduction in Yeast
Authors:  Jane E. Dorweiler and Anita L. Manogaran, date: 09/05/2021, view: 715, Q&A: 0

Cytoduction, and a related technique referred to as plasmiduction, have facilitated substantial advancements in the field of yeast prion biology by providing a streamlined method of transferring prions from one yeast strain to another. Prions are

...
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Synchronized Real-time Measurement of Sec-mediated Protein Translocation
Authors:  Riti Gupta, Dmitri Toptygin and Christian M. Kaiser, date: 08/20/2021, view: 657, Q&A: 0

The Sec translocon, consisting of a heterotrimeric transmembrane channel (SecYEG) and an associated ATPase (SecA), catalyzes the export of unfolded proteins from the cytosol in bacteria. Kinetically resolving protein translocation at high resolution

...
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