José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Adison Wong Singapore Institute of Technology
1 protocol

Beatrice Li University of Oxford
7 protocols

Changyi Zhang Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
4 protocols

Cristina Colomer-Winter Boehringer Ingelheim
2 protocols

Editor
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics molecular biology microorganisms clinical pathogens bacteria yeast
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid, 2016

Lab information

Biotecnología microbiana UFV
José Berenguer's lab: Biotechnology of thermophilic microorganisms CBMSO
http://www.ufv.es/biotecnologia-microbiana http://web4.cbm.uam.es/joomla-rl/index.php/en/scientific-departments/virology-and-microbiology?id=%20718

Publications

2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 6307, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 6177, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
4 Protocols reviewed
Induction of Natural Competence in Genetically-modified Lactococcus lactis
Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 3372, Q&A: 0
Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence ...
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Multiple Stepwise Gene Knockout Using CRISPR/Cas9 in Escherichia coli
Authors:  Enrico König, Francesca Zerbini, Ilaria Zanella, Davide Fraccascia and Guido Grandi, date: 01/20/2018, view: 16532, Q&A: 1
With the recent implementation of the CRISPR/Cas9 technology as a standard tool for genome editing, laboratories all over the world are undergoing one of the biggest advancements in molecular biology since PCR. The key advantage of this method is ...
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11 Protocols edited
Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter
Authors:  Balaji T. Moorthy, Akhilesh Kumar, Lauren X. Lotenfoe and Fangliang Zhang, date: 05/20/2020, view: 761, Q&A: 0
In the last decade, genome editing has been the center of attention as a novel tool for mechanistic investigations and for potential clinical applications. Various genome editing tools like meganucleases, zinc finger nucleases (ZFNs), transcription ...
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Conjugation Protocol Optimised for Roseburia inulinivorans and Eubacterium rectale
Authors:  Paul O. Sheridan, Jennifer C. Martin and Karen P. Scott, date: 04/05/2020, view: 454, Q&A: 0
Roseburia and Eubacterium species of the human gut microbiota play an important role in the maintaince of human health, partly by producing butyrate, the main energy source of our colonic epithelial cells. However, our knowledge of ...
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