José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Adison Wong Singapore Institute of Technology
1 protocol

Beatrice Li University of Oxford
7 protocols

Changyi Zhang Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
4 protocols

Chao Jiang Zhejiang University
8 protocols

Editor
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics molecular biology microorganisms clinical pathogens bacteria yeast
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid, 2016

Lab information

Biotecnología microbiana UFV
José Berenguer's lab: Biotechnology of thermophilic microorganisms CBMSO
http://www.ufv.es/biotecnologia-microbiana http://web4.cbm.uam.es/joomla-rl/index.php/en/scientific-departments/virology-and-microbiology?id=%20718

Publications

2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 6732, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 6559, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
5 Protocols reviewed
Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 100, Q&A: 0
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. ...
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Induction of Natural Competence in Genetically-modified Lactococcus lactis
Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 3707, Q&A: 0
Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence ...
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13 Protocols edited
A SsrA/NIa-based Strategy for Post-Translational Regulation of Protein Levels in Gram-negative Bacteria
Authors:  Gonzalo Durante-Rodríguez, Belén Calles, Víctor de Lorenzo and Pablo I. Nikel, date: 07/20/2020, view: 503, Q&A: 0
Strategies to control the levels of key enzymes of bacterial metabolism are commonly based on the manipulation of gene of interest within the target pathway. The development of new protocols towards the manipulation of biochemical processes is still ...
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Gas Chromatography Detection Protocol of Short-chain Fatty Acids in Mice Feces
Authors:  Chaozheng Zhang, An Liu, Tianshuang Zhang, Yi Li and Hua Zhao, date: 07/05/2020, view: 811, Q&A: 0
Short-chain fatty acids (SCFAs), which are formed mainly by bacteria fermenting undigested carbohydrates in the colon, they are based on the number of carbon atoms in the carbon chain. Organic fatty acids with less than 6 carbon atoms are called ...
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