José Berenguer Department of Molecular Biology, Centro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid - Consejo Superior de Investigaciones Científicas, Spain
2 protocols
Adison Wong Singapore Institute of Technology
1 protocol

Alvaro Galvis University of Nevada Las Vegas
3 protocols

Beatrice Li University of Oxford
7 protocols

Changyi Zhang Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign
6 protocols

Editor
Alba Blesa
  • Research scientist, Universidad Francisco de Vitoria
Research focus
  • Microbiology
  • thermophiles HGT antibiotics molecular biology microorganisms clinical pathogens bacteria yeast
  • 2 Author merit

Education

PhD, Universidad Autónoma de Madrid, 2016

Lab information

Biotecnología microbiana UFV
José Berenguer's lab: Biotechnology of thermophilic microorganisms CBMSO
http://www.ufv.es/biotecnologia-microbiana http://web4.cbm.uam.es/joomla-rl/index.php/en/scientific-departments/virology-and-microbiology?id=%20718

Publications

2 Protocols published
Transformation of Thermus Species by Natural Competence
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 7324, Q&A: 0
Many Thermus species harbour genomes scourged with horizontally transferred signatures. Thermus thermophilus (Tth) has been characterized as naturally competent. The transformation protocol described here is based on the ...
Cell-to-cell DNA Transfer among Thermus Species
Authors:  Alba Blesa and José Berenguer, date: 11/20/2016, view: 7118, Q&A: 0
The ability to transfer DNA via direct cell-to-cell contact-dependent process similar to conjugation has been described in Thermus thermophilus (Tth). Here, we detail the mating experiment protocol involving the lateral transfer of ...
5 Protocols reviewed
Evaluation of Viable Cells in Pseudomonas aeruginosa Biofilms by Colony Count and Live/Dead Staining
Authors:  Magdalena Pezzoni, Ramón Augusto Pizarro and Cristina Susana Costa, date: 09/20/2020, view: 1690, Q&A: 0
Pseudomonas aeruginosa is a human pathogen capable to form robust biofilms. P. aeruginosa biofilms represent a serious problem because of the adverse effects on human health and industry, from sanitary and economic points of view. ...
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Induction of Natural Competence in Genetically-modified Lactococcus lactis
Authors:  Joyce Mulder, Michiel Wels, Oscar P. Kuipers, Michiel Kleerebezem and Peter A. Bron, date: 07/05/2018, view: 4306, Q&A: 0
Natural competence can be activated in Lactoccocus lactis subsp lactis and cremoris upon overexpression of ComX, a master regulator of bacterial competence. Herein, we demonstrate a method to activate bacterial competence ...
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19 Protocols edited
Candida Biofilm Formation Assay on Essential Oil Coated Silicone Rubber

Development of biofilm associated candidemia for patients with implanted biomaterials causes an urgency to develop antimicrobial and biofilm inhibitive coatings in the management of recalcitrant Candida infections. Recently, there is an increase in

...
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Bacterial Conjugation Protocol for Ruminant Mycoplasmas
Authors:  Eveline Sagné, Christine Citti and Emilie Dordet-Frisoni, date: 01/20/2021, view: 557, Q&A: 0

In Mycoplasma agalactiae, two simultaneous processes of DNA transfer have been described that require direct cell-to-cell contact and are similar to conjugation. One involves the self-transmission of an integrative conjugative element (ICE) while

...
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