Neuroscience

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    Protocols in Current Issue
    A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord
    Authors:  Pooja Shree Mishra and Trichur R Raju, date: 01/20/2020, view: 54, Q&A: 0
    Investigations into glial biology have contributed substantially in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo present significant ...
    Protocol for Measuring Free (Low-stress) Exploration in Rats
    Authors:  Wojciech Pisula and Klaudia Modlinska, date: 01/20/2020, view: 32, Q&A: 0
    Research on exploratory behavior plays a key part in behavioral science. Studying exploratory behavior of laboratory rodents may provide important data about many developmental and neurobiological processes occurring in animal ontogenesis. The ...
    Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
    Authors:  W. Michael Babinchak and Witold K. Surewicz, date: 01/20/2020, view: 26, Q&A: 0
    Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative ...
    A Single Test to Study Social Behavior and Repetitive Self-grooming in Mice
    Authors:  Niraj V. Lawande, Ammar L. Ujjainwala and Catherine A. Christian, date: 01/20/2020, view: 55, Q&A: 0
    The ability to recognize and interact with members of the same species is essential for social communication. Investigating the neural substrates of social interest and recognition may offer insights into the behavioral differences present in ...
    A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord
    Authors:  Pooja Shree Mishra and Trichur R Raju, date: 01/20/2020, view: 54, Q&A: 0
    [Abstract] Investigations into glial biology have contributed substantially in understanding the physiology and pathology of the nervous system. However, intricacies of the neuron-glial and glial-glial interactions in vivo present significant challenges while delineating the individual cell-type contributions, thus making the in vitro ...
    Protocol for Measuring Free (Low-stress) Exploration in Rats
    Authors:  Wojciech Pisula and Klaudia Modlinska, date: 01/20/2020, view: 32, Q&A: 0
    [Abstract] Research on exploratory behavior plays a key part in behavioral science. Studying exploratory behavior of laboratory rodents may provide important data about many developmental and neurobiological processes occurring in animal ontogenesis. The proposed protocol for measuring the free (low-stress) exploration behavior in rats is straightforward, ...
    Studying Protein Aggregation in the Context of Liquid-liquid Phase Separation Using Fluorescence and Atomic Force Microscopy, Fluorescence and Turbidity Assays, and FRAP
    Authors:  W. Michael Babinchak and Witold K. Surewicz, date: 01/20/2020, view: 26, Q&A: 0
    [Abstract] Liquid-liquid phase separation (LLPS) underlies the physiological assembly of many membrane-less organelles throughout the cell. However, dysregulation of LLPS may mediate the formation of pathological aggregates associated with neurodegenerative diseases. Here, we present complementary experimental approaches to study protein aggregation within ...
    A Single Test to Study Social Behavior and Repetitive Self-grooming in Mice
    Authors:  Niraj V. Lawande, Ammar L. Ujjainwala and Catherine A. Christian, date: 01/20/2020, view: 55, Q&A: 0
    [Abstract] The ability to recognize and interact with members of the same species is essential for social communication. Investigating the neural substrates of social interest and recognition may offer insights into the behavioral differences present in disorders affecting social behavior. Assays used to study social interest in rodents include the 3-chamber ...
    Assessing Rough-and-tumble Play Behavior in Juvenile Rats
    Authors:  Jonathan W. VanRyzin, Ashley E. Marquardt and Margaret M. McCarthy, date: 01/05/2020, view: 271, Q&A: 0
    [Abstract] Play is a complex social behavior that is highly conserved across mammals. In most species, males engage in more frequent and vigorous play as juveniles than females, which reflects subtle yet impactful sex differences in brain circuitry and development. In this protocol, we describe a behavioral testing paradigm to assess social play in male and ...
    Laser Capture Micro-dissection (LCM) of Neonatal Mouse Forebrain for RNA Isolation
    Authors:  Achira Roy, Mei Deng, Kimberly A. Aldinger, Ian A. GLass and Kathleen J. Millen, date: 01/05/2020, view: 239, Q&A: 0
    [Abstract] Precise and reproducible isolation of desired cell types or layers from heterogeneous tissues is crucial to analyze specific gene profiles and molecular interactions in vivo. Forebrain is the core site of higher functions, like cognition and memory consolidation. It is composed of heterogeneous and distinct cell types, interconnected to ...
    The Mouse Gambling Task: Assessing Individual Decision-making Strategies in Mice
    Authors:  Elsa Pittaras, Arnaud Rabat and Sylvie Granon, date: 01/05/2020, view: 164, Q&A: 0
    [Abstract] Decision-making is a complex cognitive process which consists of choosing one option among several alternatives. In humans, this process is featured in the Iowa gambling task (IGT), a decision-making task that mimics real life situations by reproducing uncertain conditions based on probabilistic rewards or penalties (see Background). Several ...
    Retina Injury and Retina Tissue Preparation to Study Regeneration in Zebrafish
    Authors:  Poonam Sharma and Rajesh Ramachandran, date: 12/20/2019, view: 309, Q&A: 0
    [Abstract] Unlike mammals, primitive vertebrates have immense capability to regenerate almost all of their organs including the central nervous system. Among primitive organisms, zebrafish have been extensively used as a model system for regeneration studies. The retina is a part of the central nervous system and mammals lack the potential to repair any ...
    Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells
    Authors:  Victoria Riccio, Miluska Vissa, Angus McQuibban and Peter Kijun Kim, date: 12/20/2019, view: 561, Q&A: 0
    [Abstract] The process of autophagy is an essential cellular mechanism, required to maintain general cell health through the removal of dysfunctional organelles, such as the ER, peroxisomes and mitochondria, as well as protein aggregates, and bacteria. Autophagy is an extremely dynamic process, and tools are constantly being developed to study the various ...
    Myelin Oligodendrocyte Glycoprotein 35-55 (MOG 35-55)-induced Experimental Autoimmune Encephalomyelitis: A Model of Chronic Multiple Sclerosis
    Authors:  Sakie Miyamura, Nagisa Matsuo, Kazuki Nagayasu, Hisashi Shirakawa and Shuji Kaneko, date: 12/20/2019, view: 348, Q&A: 0
    [Abstract] Multiple sclerosis (MS) is the common demyelinating disease of human central nervous system. Among mouse models available to study MS, including the cuprizone application and lysolecithin-injection models, experimental autoimmune encephalomyelitis (EAE) model is widely used so that chronic EAE model of C57BL/6J can reflect the autoimmune ...



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