Neuroscience

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    Protocols in Current Issue
    Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
    Authors:  Yon Ju Ji and Jiou Wang, date: 01/05/2019, view: 176, Q&A: 0
    Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious ...
    Estimation of the Readily Releasable Synaptic Vesicle Pool at the Drosophila Larval Neuromuscular Junction
    Authors:  Pragya Goel, Xiling Li and Dion Dickman, date: 01/05/2019, view: 200, Q&A: 0
    Presynaptic boutons at nerve terminals are densely packed with synaptic vesicles, specialized organelles for rapid and regulated neurotransmitter secretion. Upon depolarization of the nerve terminal, synaptic vesicles fuse at specializations called ...
    Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
    Authors:  Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo, date: 01/05/2019, view: 169, Q&A: 0
    In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon ...
    Heterochronic Phenotype Analysis of Hypodermal Seam Cells in Caenorhabditis elegans
    Authors:  Yon Ju Ji and Jiou Wang, date: 01/05/2019, view: 176, Q&A: 0
    [Abstract] Heterochrony refers to changes in the timing of developmental events, and it is precisely regulated in the organisms by the heterochronic genes such as C. elegans lin-4 and let-7. Mutations in these genes cause precocious or retarded development of certain cell lineages. With well-defined cell lineages, C. elegans ...
    Estimation of the Readily Releasable Synaptic Vesicle Pool at the Drosophila Larval Neuromuscular Junction
    Authors:  Pragya Goel, Xiling Li and Dion Dickman, date: 01/05/2019, view: 200, Q&A: 0
    [Abstract] Presynaptic boutons at nerve terminals are densely packed with synaptic vesicles, specialized organelles for rapid and regulated neurotransmitter secretion. Upon depolarization of the nerve terminal, synaptic vesicles fuse at specializations called active zones that are localized at discrete compartments in the plasma membrane to initiate synaptic ...
    Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe
    Authors:  Farzane Sivandzade, Aditya Bhalerao and Luca Cucullo, date: 01/05/2019, view: 169, Q&A: 0
    [Abstract] In recent years, fluorescent dyes have been frequently used for monitoring mitochondrial membrane potential to evaluate mitochondrial viability and function. However, the reproducibility of the results across laboratories strongly depends upon following well validated and reliable protocols along with the appropriate controls. Herein, we provide a ...
    Three-chamber Social Approach Task with Optogenetic Stimulation (Mice)
    Authors:  Celeste Leung, Jun Chul Kim and Zhengping Jia, date: 12/20/2018, view: 193, Q&A: 0
    [Abstract] The formation of social relationships via social interactions and memory are essential for one’s physical and mental health. To date, rodent studies have used the three-chamber social approach test to measure social approach, social novelty, and social memory. In recent years, techniques including optogenetics have been developed to acutely ...
    Partial Transection of Adult Rat Optic Nerve as a Model of Secondary Degeneration in the Central Nervous System
    Authors:  Carole A Bartlett and Melinda Fitzgerald, date: 12/20/2018, view: 246, Q&A: 0
    [Abstract] Injury to the central nervous system is characterized by damage that spreads from the initial point of impact into the surrounding adjacent tissue, in a phenomenon referred to as secondary degeneration. The optic nerve can be used to effectively model injury and secondary degeneration to white matter tracts. Partial transection of the dorsal ...
    Purification of Soluble Recombinant Human Tau Protein from Bacteria Using Double-tag Affinity Purification
    Authors:  Joseph McInnes, Lujia Zhou and Patrik Verstreken, date: 11/20/2018, view: 508, Q&A: 0
    [Abstract] Dysfunction of the microtubule-associated protein Tau (encoded by the MAPT gene) has been implicated in more than twenty neurodegenerative diseases, including Alzheimer’s. As such, the physiological and disease-relevant functions of Tau have garnered great interest in the research community. One barrier hampering investigations into the ...
    Collagenase-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry
    Authors:  Cathrin J. Czupalla, Hanadie Yousef, Tony Wyss-Coray and Eugene C. Butcher, date: 11/20/2018, view: 520, Q&A: 0
    [Abstract] The brain endothelium is a highly specialized vascular structure that maintains the activity and integrity of the central nervous system (CNS). Previous studies have reported that the integrity of the brain endothelium is compromised in a plethora of neuropathologies. Therefore, it is of particular interest to establish a method that enables ...
    Papain-based Single Cell Isolation of Primary Murine Brain Endothelial Cells Using Flow Cytometry
    Authors:  Hanadie Yousef, Cathrin J Czupalla, Davis Lee, Eugene C Butcher and Tony Wyss-Coray, date: 11/20/2018, view: 482, Q&A: 0
    [Abstract] Brain endothelial cells (BECs) form the integral component of the blood-brain barrier (BBB) which separates the systemic milieu from the brain parenchyma and protects the brain from pathogens and circulating factors. In order to study BEC biology, it was of particular interest to establish a method that enables researchers to investigate and ...
    A Modified Barnes Maze for Juvenile Rats
    Authors:  Nazanin Valibeigi, Daniel G. McHail, Carmen L. Kimball and Theodore C. Dumas, date: 11/20/2018, view: 429, Q&A: 0
    [Abstract] To better understand neural codes for spatial navigation and to address cognitive development questions, the neurobiology of postnatal hippocampal development is receiving increased attention. While the Morris Water Maze is the gold standard for assessing hippocampal integrity, potential confounds can be difficult to control for in juvenile ...
    Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
    Authors:  Cindy L. Linn, Sarah E. Webster and Mark K. Webster, date: 11/05/2018, view: 280, Q&A: 0
    [Abstract] Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate vehicles. Here, we introduce an eye drop procedure to deliver a drug (PNU-282987), in combination ...



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