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    FRAP: A Powerful Method to Evaluate Membrane Fluidity in Caenorhabditis elegans
    Authors:  Ranjan Devkota and Marc Pilon, date: 07/05/2018, view: 355, Q&A: 0
    [Abstract] FRAP (Fluorescence Recovery After Photobleaching) is probably the most direct method to investigate the dynamics of molecules in living cells. Here, we describe FRAP to quantify membrane fluidity in C. elegans. Using FRAP, we have shown that cold, glucose and exogenous saturated fatty acids can decrease the fluidity of cellular membranes ...
    Measuring Spatiotemporal Dynamics of Odor Gradient for Small Animals by Gas Chromatography
    Authors:  Akiko Yamazoe-Umemoto, Yuishi Iwasaki and Koutarou D. Kimura, date: 04/05/2018, view: 1068, Q&A: 0
    [Abstract] Odor is the most fundamental chemical stimulus that delivers information regarding food, mating partners, enemies, and danger in the surrounding environment. Research on odor response in animals is widespread, although studies on experimental systems in which the gradient of odor concentration is quantitatively measured has been quite limited. ...
    Measurement of ROS in Caenorhabditis elegans Using a Reduced Form of Fluorescein
    Authors:  Shaarika Sarasija and Kenneth R. Norman, date: 04/05/2018, view: 857, Q&A: 0
    [Abstract] Oxidative stress is implicated in the pathogenesis of various neurodegenerative diseases, including Alzheimer’s disease. Oxidative stress is a result of a disruption of the equilibrium between antioxidants and oxidants, in favor of oxidants. Since mitochondria are major sites of production and reduction of reactive oxygen species (ROS), ...
    Analysis of Mitochondrial Structure in the Body Wall Muscle of Caenorhabditis elegans
    Authors:  Shaarika Sarasija and Kenneth R. Norman, date: 04/05/2018, view: 969, Q&A: 0
    [Abstract] Mitochondrial function is altered in various pathologies, highlighting the crucial role mitochondria plays in maintaining cellular homeostasis. Mitochondrial structure undergoes constant fission and fusion in response to changing cellular environment. Due to this, analyzing mitochondrial structure could provide insight into the physiological state ...
    In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
    [Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
    Measurement of Intracellular ROS in Caenorhabditis elegans Using 2’,7’-Dichlorodihydrofluorescein Diacetate
    Authors:  Dong Suk Yoon, Myon-Hee Lee and Dong Seok Cha, date: 03/20/2018, view: 1442, Q&A: 0
    [Abstract] Reactive oxygen species (ROS) are generated during normal metabolic processes under aerobic conditions. Since ROS production initiates harmful radical chain reactions on cellular macromolecules, including lipid peroxidation, DNA mutation, and protein denaturation, it has been implicated in a wide spectrum of diseases such as cancer, ...
    Synthetic Genetic Interaction (CRISPR-SGI) Profiling in Caenorhabditis elegans
    Authors:  John A. Calarco and Adam D. Norris, date: 03/05/2018, view: 1019, Q&A: 0
    [Abstract] Genetic interaction screens are a powerful methodology to establish novel roles for genes and elucidate functional connections between genes. Such studies have been performed to great effect in single-cell organisms such as yeast and E. coli (Schuldiner et al., 2005; Butland et al., 2008; Costanzo et al., 2010), ...
    Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
    Authors:  Yusuke Tomina and Daniel A. Wagenaar, date: 03/05/2018, view: 899, Q&A: 0
    [Abstract] In this protocol, we introduce an effective method for voltage-sensitive dye (VSD) loading and imaging of leech ganglia as used in Tomina and Wagenaar (2017). Dissection and dye loading procedures are the most critical steps toward successful whole-ganglion VSD imaging. The former entails the removal of the sheath that covers neurons in the ...
    Adapting the Smart-seq2 Protocol for Robust Single Worm RNA-seq
    [Abstract] Most nematodes are small worms that lack enough RNA for regular RNA-seq protocols without pooling hundred to thousand of individuals. We have adapted the Smart-seq2 protocol in order to sequence the transcriptome of an individual worm. While developed for individual Steinernema carpocapsae and Caenorhabditis elegans larvae as ...
    Plate Assay to Determine Caenorhabditis elegans Response to Water Soluble and Volatile Chemicals
    Authors:  Takashi Murayama and Ichiro N. Maruyama, date: 02/20/2018, view: 1062, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is widely used for behavioral studies ranging from simple chemosensation to associative learning and memory. It is vital for such studies to determine optimal concentrations of attractive and aversive chemicals that C. elegans can sense. Here we describe a resource localization assay in which a ...