Production and Isolation of Magnetic Protein Crystals in HEK293T Cells
Authors: Thomas L. Li and
Bianxiao Cui,
date: 07/20/2020,
view: 458,
Q&A: 0
[Abstract] Advances in protein engineering have enabled the production of self-assembled protein crystals within living cells. Our recent publication demonstrates the production of ftn-PAK4, which is a ferritin-containing crystal that can mineralize iron and become magnetic when isolated. We have developed an optimized protocol for the production and ...
Preparation of Yeast tRNA Sample for NMR Spectroscopy
[Abstract] Transfer RNAs (tRNAs) are heavily decorated with post-transcriptional modifications during their biosynthesis. To fulfil their functions within cells, tRNAs undergo a tightly controlled biogenesis process leading to the formation of mature tRNAs. In addition, functions of tRNAs are often modulated by their modifications. Although the biological ...
Purification of Rice Stripe Virus
Authors: Gang Lu,
Min Yao,
Yijun Zhou and
Xiaorong Tao,
date: 03/20/2020,
view: 698,
Q&A: 0
[Abstract] Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice tissues. RSV virions with high infectivity were first precipitated ...
Evaluating Whole Blood Clotting in vitro on Biomaterial Surfaces
Authors: Roberta M Sabino and
Ketul C Popat,
date: 02/05/2020,
view: 1440,
Q&A: 0
[Abstract] Biomaterial-associated thrombosis is still a major concern for blood-contacting implants. After the medical device is implanted and comes in contact with blood, several complex reactions occur, which may lead to thrombus formation and failure of the device. Therefore, it is essential to evaluate the biomaterial interaction with the whole blood. ...