Protocols in Current Issue
    In vitro Cell Wall Stress Assay for Fusarium oxysporum
    Authors:  Elena Pérez-Nadales and Antonio Di Pietro, date: 09/05/2016, view: 5845, Q&A: 0
    [Abstract] In this protocol we describe a cell wall stress assay for the fungal pathogen F. oxysporum, based on exposure to the two anionic dyes Calcofluor White (CFW) and Congo Red (CR). Both compounds have been used to exert stress upon the fungal cell wall in vitro (Perez-Nadales and Di Pietro, 2015; Perez-Nadales and Di Pietro, 2011; ...
    Quantification of Chitinase Activity in Fusarium oxysporum
    Authors:  Carmen Ruiz-Roldan and M. Isabel G. Roncero, date: 08/05/2016, view: 7222, Q&A: 1
    [Abstract] Fungal morphogenetic development requires modification and plasticity of the cell wall, which implies synthesis and remodelling of its components, including chitin and glucan. Thus chitinase and glucanase activities are crucial for cell-wall biogenesis and cell division. Quantification of chitinase activity might be useful to identify structural ...
    Determination of Intracellular ATP Levels in Mycelium of Fusarium oxysporum
    Authors:  Carmen Ruiz-Roldan and M. Isabel G. Roncero, date: 07/20/2016, view: 5377, Q&A: 0
    [Abstract] Glycolysis provides metabolites for energy production via oxidative phosphorylation during vegetative growth of Fusarium oxysporum. Therefore, determination of intracellular ATP levels might be of valuable help to analyze regulation of glycolysis/gluconeogenesis pathways. The protocol described here can be applied to other filamentous ...

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