Protocols in Current Issue
    Establishment of Patient-Derived Xenografts in Mice
    Authors:  Dongkyoo Park, Dongsheng Wang, Guo Chen and Xingming Deng, date: 11/20/2016, view: 8315, Q&A: 0
    [Abstract] Patient-derived xenograft (PDX) models for cancer research have recently attracted considerable attention in both the academy and industry (Hidalgo et al., 2014; Wilding and Bodmer, 2014). PDX models have been developed from different tumor types including lung cancer to improve the drug development process. These models are used for ...
    Phagocytosis Assay to Measure Uptake of Necroptotic Cancer Cells by BMDCs
    Authors:  Tania Løve Aaes, Dmitri V. Krysko and Peter Vandenabeele, date: 11/05/2016, view: 6642, Q&A: 0
    [Abstract] This protocol is a flow cytometry-based method to measure the phagocytosis efficiency of necroptotic target cells by bone marrow-derived dendritic cells (BMDCs) in vitro (Aaes et al., 2016). The method is a slightly modified and updated version of the protocols used in previously published papers (Krysko et al., 2006; ...
    High Fat Diet-induced Breast Cancer Model in Rat
    Authors:  Meng Ju Wu and Chun Ju Chang, date: 07/05/2016, view: 4905, Q&A: 0
    [Abstract] Obesity has been linked to breast cancer progression but the underlying mechanisms remain obscure. Being overweight or obese for a woman at the time she is diagnosed with breast cancer is linked to a high risk of recurrence regardless of treatment factors. In rodents, high body weight is also associated with increased incidence of spontaneous and ...
    In vivo Bioluminescence Imaging of Luciferase-labeled Cancer Cells
    [Abstract] Over the past decade, in vivo bioluminescent imaging has emerged as a non-invasive and sensitive tool for studying ongoing biological processes within living organisms (Contag et al., 1997; Contag et al., 1998). Based on the detection and quantitation of the photons produced by the oxidation of luciferin by luciferase ...
    Stable Isotope Resolved Metabolomics Studies in ex vivo TIssue Slices
    Authors:  Teresa W-M. Fan, Andrew N. Lane and Richard M. Higashi, date: 02/05/2016, view: 5922, Q&A: 0
    [Abstract] An important component of this methodology is to assess the role of the tumor microenvironment on tumor growth and survival. To tackle this problem, we have adapted the original approach of Warburg (Warburg, 1923), by combining thin tissue slices with Stable Isotope Resolved Metabolomics (SIRM) to determine detailed metabolic activity of human ...
    Capturing the Driving Role of Tumor-host Crosstalk in a Dynamical Model of Tumor Growth
    Authors:  Sebastien Benzekry, Afshin Beheshti, Philip Hahnfeldt and Lynn Hlatky, date: 11/05/2015, view: 5485, Q&A: 0
    [Abstract] In 1999, Hahnfeldt et al. proposed a mathematical model for tumor growth as dictated by reciprocal communications between tumor and its associated vasculature, introducing the idea that a tumor is supported by a dynamic, rather than a static, carrying capacity. In this original paper, the carrying capacity was equated with the variable ...
    Adoptive Transfer of Myeloid-Derived Suppressor Cells and T Cells in a Prostate Cancer Model
    Authors:  Libo Yan and Yan Xu, date: 08/20/2015, view: 5609, Q&A: 0
    [Abstract] The adoptive transfer of immune cells for cancer, chronic infection, and autoimmunity is an emerging field that has shown promise in recent trials. The transgenic adenocarcinoma mouse prostate (TRAMP) is a classical mouse model of prostate cancer (PCa) and TRAMP cell lines were derived from a TRAMP mouse tumor. TRAMP-C2 is tumorigenic when ...

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