Mammalia

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    Protocols in Current Issue
    In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
    Authors:  Mahdia Benkhoucha, Nicolas Molnarfi, Elodie Belnoue, Madiha Derouazi and Patrice H Lalive, date: 11/20/2018, view: 1275, Q&A: 0
    [Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

    Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
    Tumorigenicity Assay in Nude Mice
    Authors:  Feng Du, Xiaodi Zhao and Daiming Fan, date: 07/05/2017, view: 8931, Q&A: 0
    [Abstract] Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of ...
    Determining Leukocyte Origins Using Parabiosis in the PyMT Breast Tumor Model
    Authors:  Ruth A. Franklin and Ming O. Li, date: 08/20/2015, view: 5815, Q&A: 0
    [Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic ...
    Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting
    Authors:  Nicoletta Cordani, Viviana Pisa, Laura Pozzi and Clara Sciorati, date: 11/20/2014, view: 8030, Q&A: 1
    [Abstract] A population of muscle resident CD45-, CD31- cells expressing the mesenchymal PDGF receptor alpha (PDGFRα) as well as Sca-1 was first isolated in healthy mouse muscles in Uezumi et al. (2010). In the same year, Joe et al. (2010) identified and purified fibro-adipogenic precursors (FAPs), cells located into ...
    Isolation and in vivo Transfer of Antigen Presenting Cells
    Authors:  Pooja Arora, Shalu Sharma Kharkwal and Steven A. Porcelli, date: 10/05/2014, view: 7706, Q&A: 0
    [Abstract] Transfer of antigen presenting cells in vivo is a method used by immunologists to examine the potency of antigen presentation by a selected population of cells. This method is most commonly used to analyze presentation of protein antigens to MHC class I or II restricted T cells, but it can also be used for studies of nonconventional ...
    Vaccine-induced Cytokine Production Detected by Luminex Multiplex Analysis
    [Abstract] Different vaccine and adjuvant combinations are known to rapidly induce antigen presenting cell (APC) maturation and pro-inflammatory cytokine and production, which in turn play an important role in the priming of antigen-specific T cells. Measuring cytokine production systemically in the serum fails to detect localized responses in the lymph ...



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