Mammalia

Categories

    Protocols in Current Issue
    Extraction of DNA from Murine Fecal Pellets for Downstream Phylogenetic Microbiota Analysis by Next-generation Sequencing
    Authors:  Elien Eeckhout and Andy Wullaert, date: 02/05/2018, view: 5332, Q&A: 2
    [Abstract] Mouse models are widely used to evaluate the potential impact of the gut microbial composition on health and disease. Standardized protocols for sampling and storing murine feces, as well as for extracting DNA from these fecal pellets are needed to limit experimental variation between different studies. Both efficient lysis of the microbiota and ...
    Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
    Authors:  Ran Wang and Sumaira Z. Hasnain, date: 07/20/2017, view: 10341, Q&A: 0
    [Abstract] Specialized secretory cells known as goblet cells in the intestine and respiratory epithelium are responsible for the secretion of mucins. Mucins are large heavily glycosylated proteins and typically have a molecular mass higher than 106 Da. These large proteins are densely substituted with short glycan chains, which have many important ...
    Spore Preparation Protocol for Enrichment of Clostridia from Murine Intestine
    Authors:  Eric M. Velazquez, Fabian Rivera-Chávez and Andreas J. Bäumler, date: 05/20/2017, view: 4254, Q&A: 0
    [Abstract] In recent years, many spore-forming commensal Clostridia found in the gut have been discovered to promote host physiology, immune development, and protection against infections. We provide a detailed protocol for rapid enrichment of spore-forming bacteria from murine intestine. Briefly, contents from the intestinal cecum are collected aerobically, ...
    Mouse Model of Reversible Intestinal Inflammation
    [Abstract] Current therapies to treat inflammatory bowel disease by dampening excessive inflammatory immune responses have had limited success (Reinisch et al., 2011; Rutgeerts et al., 2005; Sandborn et al., 2012). To develop new therapeutic interventions, there is a need for better understanding of the mechanisms that are ...
    Isolation of Intestinal Mesenchymal Cells from Adult Mice
    Authors:  Vasiliki Koliaraki and George Kollias, date: 09/20/2016, view: 12192, Q&A: 0
    [Abstract] During the last 20 years intestinal mesenchymal cells (IMCs) have emerged as an important cell type that plays a central role in intestinal development and homeostasis, by providing both structural support and growth regulatory elements. IMCs also actively participate in wound healing responses, thus regulating pathologic conditions such as tissue ...
    Analysis of Enteric Neural Crest Cell Migration Using Heterotopic Grafts of Embryonic Guts
    Authors:  Rodolphe Soret and Nicolas Pilon, date: 09/05/2016, view: 4757, Q&A: 0
    [Abstract] Hirschsprung disease (HSCR), also named aganglionic megacolon, is a severe congenital malformation characterized by a lack of enteric nervous system (ENS) in the terminal regions of the bowel (Bergeron et al., 2013). As the ENS notably regulates motility in the whole gastrointestinal track, the segment without neurons remains tonically ...
    Immunofluorescent Staining of Mouse Intestinal Stem Cells
    Authors:  Kevin P. O’Rourke, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 22227, Q&A: 1
    [Abstract] Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, ...
    Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
    Authors:  Kevin P. O’Rourke, Sarah Ackerman, Lukas E Dow and Scott W Lowe, date: 02/20/2016, view: 17459, Q&A: 6
    [Abstract] In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild ...
    Intravenous Tomato Lectin Injection to Assess Functional Vasculature
    Authors:  Carey L Watson, Maxime M Mahe and Michael A. Helmrath, date: 10/20/2015, view: 7538, Q&A: 1
    [Abstract] Pluripotent stem cells have recently allowed for the development of tissue models for the various organ systems within the body. These models allow scientists to study organ function, physiology, embryology, and even pathologic processes. Studies on tissue can be done in vitro and/or transplanted into animal models for studies in vivo ...
    In vivo Fluorescein Isothiocyanate-dextran (FD4) Permeability Assay
    Authors:  Carey L Watson, Maxime M Mahe and Michael A. Helmrath, date: 10/20/2015, view: 13306, Q&A: 1
    [Abstract] Using pluripotent stem cells, it is now becoming possible to develop tissue models of organ systems within the body. These organs will allow for the study of organ function, physiology, embryology, and even pathologic processes. Recently, our group developed a model of human small intestine developed from human pluripotent stem cells which when ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.