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    Protocols in Current Issue
    Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling
    Authors:  Laurent Simon and Feth el Zahar Haichar, date: 05/05/2019, view: 913, Q&A: 0
    [Abstract] One of the most remarkable metabolic features of plant roots is their ability to secrete a wide range of compounds into the rhizosphere, defined as the volume of soil around living roots. Around 5%-21% of total photosynthetically fixed carbon is transferred into the rhizosphere through root exudates. Until recently, studies on the quantity and ...
    Measuring and Imaging the Soil-root-water System with a Light Transmission 2D Technique
    Authors:  Claude Doussan and Emmanuelle Garrigues, date: 03/20/2019, view: 1149, Q&A: 0
    [Abstract] Improving crops against water deficits requires a better understanding of plant root system functioning. This requires a better knowledge of the water uptake process and to address the influence of root system architecture or root physiological properties on the uptake efficiency. To this end, we describe here a non-destructive system that enables ...
    In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
    [Abstract] The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
    Quantification of Membrane Damage/Cell Death Using Evan’s Blue Staining Technique
    [Abstract] Membrane damage is a hallmark of both biotic and abiotic stress responses. The membrane determines the ability of a cell to sustain altered environmental conditions and hence can be used as a biomarker to assess stress-induced cell damage or death. We present an easy, quick, cost-effective, staining and spectrophotometric method to assess membrane ...
    Scanner-based Time-lapse Root Phenotyping
    [Abstract] Non-destructive phenotyping of root system architecture can facilitate breeding for root traits that optimize resource acquisition. This protocol describes the construction of a low-cost, high-resolution root phenotyping platform, requiring no sophisticated equipment and adaptable to most laboratory and glasshouse environments. The phenotyping ...
    Collection of Root Exudate from Duckweed
    Authors:  Yufang Lu, Li Sun and Weiming Shi, date: 01/05/2015, view: 9242, Q&A: 0
    [Abstract] Root exudates play an important role in rhizosphere interactions between plants and microorganisms. However, collection of chemicals from plant root system is difficult due to their low concentrations and high level of contaminants in growth media. The continuous collection method has been described in several terrestrial plants over the past 30 ...



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