Fish

Categories

    Protocols in Current Issue
    Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos
    Authors:  Rachna Narayanan and Andrew C. Oates, date: 03/20/2019, view: 2751, Q&A: 0
    [Abstract] In situ hybridization is used to visualize the spatial distribution of gene transcripts in tissues and in embryos, providing important information about disease and development. Current methods involve the use of complementary riboprobes incorporating non-radioactive labels that can be detected by immunohistochemistry and coupled to ...
    Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer
    Authors:  Agnik Dasgupta, Andrew E. Jacob and Jeffrey D. Amack, date: 11/20/2018, view: 1762, Q&A: 0
    [Abstract] A transient epithelial structure called the left-right organizer (LRO) establishes left-right asymmetry in vertebrate embryos. Developmental defects that alter LRO formation result in left-right patterning errors that often lead to congenital heart malformations. However, little is known about mechanisms that regulate individual cell behaviors ...
    Zebrafish Embryo Xenograft and Metastasis Assay
    Authors:  Ilkka Paatero, Sanni Alve, Silvia Gramolelli, Johanna Ivaska and Päivi M. Ojala, date: 09/20/2018, view: 3635, Q&A: 1
    [Abstract] Xenograft models, and in particular the mouse xenograft model, where human cancer cells are transplanted into immunocompromised mice, have been used extensively in cancer studies. Although these models have contributed enormously to our understanding of cancer biology, the zebrafish xenograft model offers several advantages over the mouse model. ...
    Detection of Reactive Oxygen Species Using MitoSOX and CellROX in Zebrafish
    Authors:  Alberto Rissone and Fabio Candotti, date: 10/05/2016, view: 15112, Q&A: 1
    [Abstract] The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the ...
    Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos
    Authors:  Jingli Cao, Xueliang Zhu and Xiumin Yan, date: 07/20/2014, view: 10607, Q&A: 0
    [Abstract] Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos.
    Motility Assay for Zebrafish Embryos
    Authors:  Michelle F. Goody and Clarissa A. Henry, date: 06/05/2013, view: 8753, Q&A: 0
    [Abstract] Analyzing the swimming ability of 2 days post fertilization zebrafish embryos can be a useful technique to study neuromuscular function. Here is a protocol for determining the time it takes for zebrafish embryos to swim a predetermined distance.
    Phalloidin Staining and Immunohistochemistry of Zebrafish Embryos
    Authors:  Michelle F. Goody and Clarissa A. Henry, date: 06/05/2013, view: 20436, Q&A: 1
    [Abstract] Fluorescent conjugated Phalloidin is a stain that allows for visualization of F-actin. In immunohistochemistry, primary antibodies and fluorescent conjugated secondary antibodies can be used to visualize subcellular localization and relative amounts of proteins of interest. Here is a protocol for Phalloidin and antibody staining of zebrafish ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.