Protocols in Current Issue
    Cell Wall-bound p-Coumaric and Ferulic Acid Analysis
    Author:  Nickolas Anderson, date: 10/05/2016, view: 4223, Q&A: 0
    [Abstract] Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These compounds are widely conserved in plants and primarily accumulate in the secondary cell wall. They serve as important structural components that contribute to the overall strength and rigidity of ...
    Establishment of a Fusarium graminearum Infection Model in Arabidopsis thaliana Leaves and Floral Tissues
    Authors:  Vamsi J Nalam, Sujon Sarowar and Jyoti Shah, date: 07/20/2016, view: 6979, Q&A: 0
    [Abstract] Fusarium graminearum (Fg) is the causal agent of Fusarium head blight disease of wheat (Triticum aestivum), oats (Avena sativa) and barley (Hordeum vulgare), which targets the floral tissues and thereby adversely impacts grain yield and quality. Mycotoxins produced by F. graminearum further limit the ...
    3’ Rapid Amplification of cDNA Ends (3’ RACE) Using Arabidopsis Samples
    [Abstract] Production of functional eukaryotic RNA is a very elaborate process that involves a complex interplay between transcription and various RNA processing activities, including splicing, 5’ capping, and 3’ cleavage and polyadenylation (Bentley, 2014). Accurate mapping of RNA ends provides a valuable tool to assess transcriptional and ...
    Arabidopsis thaliana Embryo Sac Mitochondrial Membrane Potential Stain
    [Abstract] The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting ...
    Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
    Authors:  Pablo Bolaños-Villegas, Xiaohui Yang, Christopher A. Makaroff and Guang-Yuh Jauh, date: 04/20/2014, view: 10402, Q&A: 0
    [Abstract] This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform ...

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