Plant Science

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    Protocols in Current Issue
    Tensile Testing Assay for the Measurement of Tissue Stiffness in Arabidopsis Inflorescence Stem
    Authors:  Kouki Yoshida, Shingo Sakamoto and Nobutaka Mitsuda, date: 08/05/2019, view: 431, Q&A: 0
    [Abstract] Lignocellulosic biomass is a versatile renewable resource for fuels, buildings, crafts, and biomaterials. Strategies of molecularly designing lignocellulose for industrial application has been developed by the discoveries of novel genes after the screenings of various mutants and transformed lines of Arabidopsis whose cell walls could be ...
    Quantification of Blumenol Derivatives as Leaf Biomarkers for Plant-AMF Association
    Authors:  Elisabeth Mindt, Ming Wang, Martin Schäfer, Rayko Halitschke and Ian T. Baldwin, date: 07/20/2019, view: 631, Q&A: 0
    [Abstract] Symbiotic interactions between arbuscular mycorrhizal fungi (AMF) and plants are widespread among land plants and can be beneficial for both partners. The plant is provided with mineral nutrients such as nitrogen and phosphorous, whereas it provides carbon resources for the fungus in return. Due to the large economic and environmental impact, ...
    An Adjustable Protocol to Analyze Chemical Profiles of Non-sterile Rhizosphere Soil
    Authors:  Alex Williams, Jurriaan Ton and Pierre Pétriacq, date: 05/20/2019, view: 1122, Q&A: 0
    [Abstract] The analysis of chemical diversity in non-sterile rhizosphere soil has been a pressing methodological challenge for years. Rhizosphere-enriched chemicals (i.e., rhizochemicals) include root exudation chemicals, (microbial) breakdown products thereof, and de novo produced metabolites by rhizosphere-inhabiting microbes, all of ...
    Tracking Root Interactions System (TRIS) Experiment and Quality Control
    Authors:  Hassan Massalha, Elisa Korenblum, Orr H. Shapiro and Asaph Aharoni, date: 04/20/2019, view: 1307, Q&A: 0
    [Abstract] Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high ...
    Quantification of the Humidity Effect on HR by Ion Leakage Assay
    Authors:  Musoki Mwimba and Xinnian Dong, date: 04/05/2019, view: 831, Q&A: 0
    [Abstract] We describe a protocol to measure the contribution of humidity on cell death during the effector-triggered immunity (ETI), the plant immune response triggered by the recognition of pathogen effectors by plant resistance genes. This protocol quantifies tissue cell death by measuring ion leakage due to loss of membrane integrity during the ...
    Peptide Feeding and Mechanical Wounding for Tomato Seedlings
    Authors:  Lei Wang, Yan Wang and Georg Felix, date: 03/20/2019, view: 1353, Q&A: 0
    [Abstract] Plants need to respond appropriately to wounding and herbivorous insects. Peptide signals have been implicated in local and systemic induction of appropriate plant defense responses. To study these peptide signals and their perception in host plants, it is important to have reproducible bioassays. Several assays, such as treatment of peptide ...
    Measuring and Imaging the Soil-root-water System with a Light Transmission 2D Technique
    Authors:  Claude Doussan and Emmanuelle Garrigues, date: 03/20/2019, view: 1187, Q&A: 0
    [Abstract] Improving crops against water deficits requires a better understanding of plant root system functioning. This requires a better knowledge of the water uptake process and to address the influence of root system architecture or root physiological properties on the uptake efficiency. To this end, we describe here a non-destructive system that enables ...
    I Plate-based Assay for Studying How Fungal Volatile Compounds (VCs) Affect Plant Growth and Development and the Identification of VCs via SPME-GC-MS
    Authors:  Wenzhao Wang, Ningxiao Li, Xingzhong Liu and Seogchan Kang, date: 02/20/2019, view: 1456, Q&A: 0
    [Abstract] Biogenic volatile compounds (VCs) mediate various types of crucial intra- and inter-species interactions in plants, animals, and microorganisms owing to their ability to travel through air, liquid, and porous soils. To study how VCs produced by Verticillium dahliae, a soilborne fungal pathogen, affect plant growth and development, we ...
    Examining Autophagy in Plant by Transmission Electron Microscopy (TEM)
    Authors:  Xiyin Zheng, Chenguang Zhao and Yule Liu, date: 10/20/2018, view: 1479, Q&A: 0
    [Abstract] In plants, macroautophagy, here referred as autophagy, is a degradation pathway during which the double-membrane structure named autophagosome engulfs the cargo and then fuses with vacuole for material recycling.

    To investigate the process of autophagy, transmission electron microscopy (TEM) was used to monitor the ultrastructure of ...
    Pneumatic Method to Measure Plant Xylem Embolism
    Authors:  Paulo R. L. Bittencourt, Luciano Pereira and Rafael S. Oliveira, date: 10/20/2018, view: 1557, Q&A: 0
    [Abstract] Embolism, the formation of air bubbles in the plant water transport system, has a major impact on plant water relations. Embolism formation in the water transport system of plants disrupts plant water transport capacity, impairing plant functioning and triggering plant mortality. Measuring embolism with traditional hydraulic methods is both ...



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