• Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011

Biochemistry

Measuring Small-molecule Inhibition of Protein Interactions in Live Cells Using FLIM-FRET Authors:  James M. Pemberton, Qian Liu and David W. Andrews, date: 10/20/2019, view: 1107, Q&A: 0
This protocol was designed to quantitatively measure small-molecule displacement of proteins in live mammalian cells using fluorescence lifetime imaging microscopy–Förster resonance energy transfer (FLIM-FRET). Tumour cell survival is often dependent on anti-apoptotic proteins, which bind to and inhibit pro-apoptotic proteins, thus preventing ...
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Cancer Biology

Immunofluorescence-based Determination of Centrosome Number in Tissue Samples Authors:  Mengdie Wang, Gregory C. Rogers and Anne E. Cress, date: 10/20/2019, view: 596, Q&A: 0
Centrosome numerical abnormalities have been reported in a variety of tumors. Centrosome numbers in cancer cells display both inter-tumor and intra-tumor heterogeneity. The over production of centrosomes (centrosome amplification) is unique in cancer cells and is a promising target for therapy. Thus, a method to quantify centrosome numbers on a ...
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Cell Biology

Phospho-protein Analysis in Adherent Cells Using Flow Cytometry Authors:  Renu Sharma, Amit Sharma, Atul Kumar and Bithiah Grace Jaganathan, date: 10/20/2019, view: 604, Q&A: 0
Protein phosphorylation is one of the most important post-translational modifications, which acts as a reversible on or off switch for the activity of a large number of proteins. Analyzing the phosphorylation status of different proteins can reveal the alterations in the state of the cells in response to cellular damage, cancer and pharmaceutical ...
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Time-lapse Imaging of Alveologenesis in Mouse Precision-cut Lung Slices Authors:  Khondoker M. Akram, Laura L.Yates, Róisín Mongey, Stephen Rothery, David C. A. Gaboriau, Jeremy Sanderson, Matthew Hind, Mark Griffiths and Charlotte H. Dean, date: 10/20/2019, view: 441, Q&A: 0
Alveoli are the gas-exchange units of lung. The process of alveolar development, alveologenesis, is regulated by a complex network of signaling pathways that act on various cell types including alveolar type I and II epithelial cells, fibroblasts and the vascular endothelium. Dysregulated alveologenesis results in bronchopulmonary dysplasia in ...
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Developmental Biology

Immunohistochemical Identification of Muscle Fiber Types in Mice Tibialis Anterior Sections Authors:  Vanitha V. Rao and Abhishek Mohanty, date: 10/20/2019, view: 251, Q&A: 0
Mammalian skeletal muscle is a metabolically active tissue that is made up of different types of muscle fibers. These myofibers are made up of important contractile proteins that provide force during contraction of the muscle like actin and myosin. Murine myofibers have been classified into 4 types: Type I, Type IIa, Type IIb and Type IIX. Each ...
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Microbiology

Quantification of HIV-2 DNA in Whole Blood Authors:  Zsófia Szojka, Sara Karlson, Marianne Jansson and Patrik Medstrand, date: 10/20/2019, view: 255, Q&A: 0
Time to AIDS infection is longer with HIV-2, compared to HIV-1, but without antiretroviral therapy both infections will cause AIDS-related mortality. In HIV-2 infection, monitoring of antiretroviral treatment (ART) efficacy is challenging since a large proportion of HIV-2-infected individuals displays low or undetectable plasma RNA levels. Hence, ...
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Probabilistic Models for Predicting Mutational Routes to New Adaptive Phenotypes Authors:  Eric Libby and Peter A. Lind, date: 10/20/2019, view: 395, Q&A: 0
Understanding the translation of genetic variation to phenotypic variation is a fundamental problem in genetics and evolutionary biology. The introduction of new genetic variation through mutation can lead to new adaptive phenotypes, but the complexity of the genotype-to-phenotype map makes it challenging to predict the phenotypic effects of ...
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Molecular Biology

Simple Synthesis of Functionalized Paramagnetic Beads for Nucleic Acid Purification and Manipulation Authors:  Phil Oberacker, Peter Stepper, Donna Bond, Katharina Hipp, Timothy A. Hore and Tomasz P. Jurkowski, date: 10/20/2019, view: 559, Q&A: 0
The purification of nucleic acids is one of the most common procedures employed in modern molecular biology laboratories. Typically, commercial column-based protocols are utilized to isolate DNA or RNA from various sources. However, these methods not only require specialized equipment, but are also extremely expensive for high-throughput ...
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Platelet Isolation and Activation Assays Authors:  Laura C. Burzynski, Nicholas Pugh and Murray C.H. Clarke, date: 10/20/2019, view: 460, Q&A: 0
Platelets regulate hemostasis and are the key determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets circulate in an inactive state, but become activated in response to damage to the endothelium, which exposes thrombogenic material such as collagen to the blood flow. Activation results in a number of responses, ...
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Neuroscience

Use of the Vsoc-maze to Study Sociability and Preference for Social Novelty in Rodents Authors:  Sara Martínez-Torres, Maria Gomis-González, Alba Navarro-Romero, Rafael Maldonado and Andrés Ozaita, date: 10/20/2019, view: 226, Q&A: 0
Studying social behavior in mouse models empowers the understanding of the neurobiological mechanisms involved, which are affected in neuropsychiatric disorders, allowing the evaluation of therapeutic strategies. Behavioral methods available are time-consuming and reducing the length of behavioral sessions may render more manageable experiments ...
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Intracerebral Injection of Streptozotocin to Model Alzheimer Disease in Rats Authors:  Daniel Moreira-Silva, Robson C. L. Vizin, Talita M. S. Martins, Tatiana L. Ferreira, Maria C. Almeida and Daniel C. Carrettiero, date: 10/20/2019, view: 367, Q&A: 0
Animal models have promoted meaningful contribution to science including Alzheimer’s disease (AD) research. Several animal models for AD have been used, most of them related to genetic mutations observed in familial AD. However, sporadic form of AD, also named late-onset is the most frequent form of the disease, which is multifactorial, being ...
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Optogenetic Food Odor Avoidance Assay Authors:  Jay M. Patel, Jessica Swanson and Benjamin R. Arenkiel, date: 10/20/2019, view: 249, Q&A: 0
Appetite is tightly linked to the sensory experience of feeding, including the smell, taste, and sight of food. Sensory perception can affect the palatability of food, modulating appetite beyond homeostatic requirements. Hypothalamic neurons that govern feeding are responsive to sensory cues associated with food, including food odors. However, the ...
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Plant Science

Cell Wall Compositional Analysis of Rice Culms Authors:  Lanjun Zhang, Baocai Zhang and Yihua Zhou, date: 10/20/2019, view: 277, Q&A: 0
The plant cell wall is a complicated network that is mainly constituted of polysaccharides, such as cellulose, hemicellulose and pectin. Many noncellulosic polysaccharides are further acetylated, which confers these polymers flexible physicochemical properties. Due to the significance of cell wall in plant growth and development, the analytic ...
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Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments of Arabidopsis Leaf Tissues Authors:  David B. Medeiros, Stéphanie Arrivault, Jessica Alpers, Alisdair R. Fernie and Fayezeh Arabi, date: 10/20/2019, view: 250, Q&A: 0
The accurate determination of metabolite distribution in subcellular compartments is still challenging in plant science. Various methodologies, such as fluorescence resonance energy transfer-based technology, nuclear magnetic resonance spectroscopy and protoplast fractionation allow the study of metabolite compartmentation. However, large changes ...
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Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings Authors:  Sandeep B. Adavi, Lekshmy Sathee, Birendra K. Padhan, Ompal Singh, Hari S. Meena, Kumar Durgesh and Shailendra K. Jha, date: 10/20/2019, view: 414, Q&A: 0
Nitric oxide (NO), is a redox-active, endogenous signalling molecule involved in the regulation of numerous processes. It plays a crucial role in adaptation and tolerance to various abiotic and biotic stresses. In higher plants, NO is produced either by enzymatic or non-enzymatic reduction of nitrite and an oxidative pathway requiring a putative ...
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