• Volume 10, 2020
  • Volume 9, 2019
  • Volume 8, 2018
  • Volume 7, 2017
  • Volume 6, 2016
  • Volume 5, 2015
  • Volume 4, 2014
  • Volume 3, 2013
  • Volume 2, 2012
  • Volume 1, 2011
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Current Issue in 2020

Volume: 10, Issue: 22

Biochemistry

Total Triglyceride Quantification in Caenorhabditis elegans Authors:  Anjali Sandhu and Varsha Singh, date: 11/20/2020, view: 650, Q&A: 0
Several studies suggest an important role of lipid metabolism in regulating longevity of Caenorhabditis elegans. Therefore, assays to quantify lipids have enormous value in understanding aging and pathologies associated with it. Approximately 70% of lipid metabolism genes in the nematode have orthologs in humans. Amenability of C. elegans ...
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Cleavable Affinity Purification (Cl-AP): A One-step Procedure to Affinity Purify Protein Complexes Authors:  Ammarah Tariq, Lucy Green, Christian Soeller and James G. Wakefield, date: 11/20/2020, view: 806, Q&A: 0

Cleavable Affinity Purification (Cl-AP) uses a tripartite system of Protein-A-Streptavidin beads and nanobodies, coupled with a biotinylated, thiol-cleavable linker, providing one-step affinity purification from lysates of tissues expressing tagged proteins. This technique allows fluorescent versions of mitotic protein complexes to be isolated

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Biophysics

Serial Cryomicrotomy of Saccharomyces cerevisiae for Serial Electron Cryotomography Authors:  Cai Tong Ng, Mark S. Ladinsky and Lu Gan, date: 11/20/2020, view: 438, Q&A: 0
Electron cryotomography (cryo-ET) is an increasingly popular technique to study cellular structures and macromolecules in situ. Due to poor penetration of electrons through thick biological samples, the vitreously frozen samples for cryo-ET need to be thin. For frozen-hydrated cells, such samples can be produced either by cryomicrotomy or ...
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Cancer Biology

Dissecting the Rat Mammary Gland: Isolation, Characterization, and Culture of Purified Mammary Epithelial Cells and Fibroblasts Authors:  Elizabeth A. Tovar, Rachael Sheridan, Curt J. Essenburg, Patrick S. Dischinger, Menusha Arumugam, Megan E. Callaghan, Carrie R. Graveel and Matthew R. Steensma, date: 11/20/2020, view: 646, Q&A: 0

With the advent of CRISPR-Cas and the ability to easily modify the genome of diverse organisms, rat models are being increasingly developed to interrogate the genetic events underlying mammary development and tumorigenesis. Protocols for the isolation and characterization of mammary epithelial cell subpopulations have been thoroughly developed for

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Magnet-assisted Flow Cytometry of in vivo Tumors to Quantitate Cell-specific Responses to Magnetic Iron Oxide Nanoparticles Authors:  Preethi Korangath and Robert Ivkov, date: 11/20/2020, view: 478, Q&A: 0
A clear understanding of nanoparticle interactions with living systems at the cellular level is necessary for developing nanoparticle-based therapeutics. Magnetic iron oxide nanoparticles provide unique opportunities to study these interactions because of their responsiveness to magnetic fields. This enables sorting of cells containing ...
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Immunology

Maternal Immune Activation with the Viral Mimetic Poly:IC in Pregnant Rats Authors:  Thaisa Meira Sandini, Quentin Greba, Brittney Rose Lins and John George Howland, date: 11/20/2020, view: 454, Q&A: 0

Maternal immune activation (MIA) is increasingly well appreciated as an environmental risk factor for some psychiatric disorders. Administration of proinflammatory compounds such as the synthetic double-stranded RNA molecule polyinosinic-polycytidylic acid (polyI:C) to pregnant rodents results in the release of proinflammatory cytokines in the

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Generating Three-dimensional Human Granulomas in vitro to Study Mycobacterium tuberculosis-host Interaction Authors:  Ainhoa Arbués, Michael Kammüller and Damien Portevin, date: 11/20/2020, view: 498, Q&A: 0
Granulomas are organized multicellular structures that constitute the hallmark of an infection by the human pathogen Mycobacterium tuberculosis (Mtb). A better understanding of the complex host-Mtb interactions within the granuloma’s environment may lead to new therapeutic or preventive tools to improve the control of the ...
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Microbiology

Rapid Isolation and Purification of Secreted Bacteriocins from Streptococcus mutans and Other Lactic Acid Bacteria Authors:  Marcus Cheng, Siew-Ging Gong and Céline M. Lévesque, date: 11/20/2020, view: 616, Q&A: 0
Bacteriocins are small ribosomally synthesized antimicrobial peptides produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for low pH. Bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. Bacteriocins produced by LAB ...
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Giant Mimiviridae CsCl Purification Protocol Authors:  Lionel Bertaux, Audrey Lartigue and Sandra Jeudy, date: 11/20/2020, view: 381, Q&A: 0
While different giant viruses’ purification protocols are available, they are not fully described and they use sucrose gradient that does not reach an equilibrium. Here, we report a protocol for the purification of members of the Mimiviridae family virions resulting from Acanthamoeaba castellanii infections. Viruses are ...
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A Quick Method for Screening Biocontrol Efficacy of Bacterial Isolates against Bacterial Wilt Pathogen Ralstonia solanacearum in Tomato Authors:  Heena Agarwal, Bhaskar Dowarah and Niraj Agarwala, date: 11/20/2020, view: 501, Q&A: 0

Ralstonia solanacearum is a bacterial phytopathogen able to cause bacterial wilt disease in more than 200 plant species. Plant disease biocontrol strategies are used for controlling this disease and tomato is used as a model plant to conduct R. solanacearum associated studies. Conventional screening methods such as seed bacterization, soil

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Neuroscience

In vitro Time-lapse Imaging of Primary Cilium in Migrating Neuroblasts Authors:  Masato Sawada, Mami Matsumoto, Keishi Narita, Natsuko Kumamoto, Shinya Ugawa, Sen Takeda and Kazunobu Sawamoto, date: 11/20/2020, view: 415, Q&A: 0
Neuronal migration is a critical step for the development of neuronal circuits in the brain. Immature new neurons (neuroblasts) generated in the postnatal ventricular-subventricular zone (V-SVZ) show a remarkable potential to migrate for a long distance at a high speed in the postnatal mammalian brain, and are thus a powerful model to analyze the ...
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Confocal Microscopy of Reovirus Transport in Living Dorsal Root Ganglion Neurons Authors:  Pavithra Aravamudhan, Krishnan Raghunathan and Terence S. Dermody, date: 11/20/2020, view: 406, Q&A: 0

Neurotropic reoviruses repurpose host machinery to traffic over long distances in neuronal processes and access distal replication sites. Understanding mechanisms of neuronal transmission is facilitated by using simplified in vitro primary neuronal culture models. Advances in the design of compartmentalized microfluidic devices lend robustness to

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Visual-looming Shadow Task with in-vivo Calcium Activity Monitoring to Assess Defensive Behaviors in Mice Authors:  Nuria Daviu, Tamas Fuzesi, David G.Rosenegger, Govind Peringod, Kathryn Simone and Jaideep S. Bains, date: 11/20/2020, view: 503, Q&A: 0
There has been a clear movement in recent years towards the adoption of more naturalistic experimental regimes for the study of behavior and its underlying neural architecture. Here we provide a protocol that allows experimenters working with mice, to mimic a looming and advancing predatory threat from the sky. This approach is easy to implement ...
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Plant Science

Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum) Authors:  Natasha Navet and Miaoying Tian, date: 11/20/2020, view: 565, Q&A: 1

Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding and deploy effective disease management strategies, and breeding through genetic engineering. Here we

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