Protocols in Current Issue
    Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
    [Abstract] The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain. First, bioluminescence signals originating from a single muscle fiber can constitute a major pitfall. Second, ...
    Measuring Behavioral Individuality in the Acoustic Startle Behavior in Zebrafish
    Authors:  Carlos Pantoja, Adam Hoagland, Elizabeth Carroll, David Schoppik and Ehud Y. Isacoff, date: 04/05/2017, view: 1267, Q&A: 0
    [Abstract] The objective of this protocol is to provide a detailed description for the construction and use of a behavioral apparatus, the zBox, for high-throughput behavioral measurements in larval zebrafish (Danio rerio). The zBox is used to measure behavior in multiple individuals simultaneously. Individual fish are housed in wells of multi-well ...
    Transplantation of Mesenchymal Cells Including the Blastema in Regenerating Zebrafish Fin
    Authors:  Eri Shibata, Kazunori Ando and Atsushi Kawakami, date: 01/20/2017, view: 1498, Q&A: 0
    [Abstract] Regeneration of fish fins and urodele limbs occurs via formation of the blastema, which is a mass of mesenchymal cells formed at the amputated site and is essential for regeneration. The blastema transplantation, a novel technique developed in our previous studies (Shibata et al., 2016; Yoshinari et al., 2012) is a useful ...
    Various Modes of Spinal Cord Injury to Study Regeneration in Adult Zebrafish
    Authors:  Subhra Prakash Hui and Sukla Ghosh, date: 12/05/2016, view: 2126, Q&A: 0
    [Abstract] Spinal cord injury (SCI) in mammals leads to failure of both sensory and motor functions, due to lack of axonal regrowth below the level of injury as well as inability to replace lost neural cells and to stimulate neurogenesis. In contrast, fish and amphibians are capable of regenerating a variety of their organs like limb/fin, jaw, heart and ...
    Allogeneic Transplantation of Testicular Hyperplasia in rag1 Mutant Zebrafish
    Authors:  Toshihiro Kawasaki and Noriyoshi Sakai, date: 11/05/2016, view: 1541, Q&A: 0
    [Abstract] Allogeneic organ transplantation is a powerful tool for clinical and basic research studies. However, the graft is often rejected by the host organism. Here, we describe a protocol that uses immunodeficient rag1 mutant zebrafish. These zebrafish escaped rejection, which made it possible to successfully transplant fragments of an ...
    Detection of Reactive Oxygen Species Using MitoSOX and CellROX in Zebrafish
    Authors:  Alberto Rissone and Fabio Candotti, date: 10/05/2016, view: 3780, Q&A: 0
    [Abstract] The production of free radicals is the result of normal cellular metabolism. Free radicals are involved in innumerable different cellular and biological functions such as signaling, proliferation, cell death, aging, inflammation, etc. Under physiological conditions, the levels of reactive oxygen species (ROS) are strictly regulated by the ...
    Thirty-Second Net Stressor Task in Adult Zebrafish
    Authors:  Steven Tran and Robert Gerlai, date: 03/05/2015, view: 2669, Q&A: 0
    [Abstract] Zebrafish have become a popular animal model for behavioral neuroscience (Gerlai, 2014). Recent studies have demonstrated that brief experimental handling prior to euthanizing animals can subsequently alter biological measures quantified post-mortem (e.g. cortisol levels) (Ramsay et al., 2009; Tran et al., 2014). Here we ...
    Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos
    Authors:  Jingli Cao, Xueliang Zhu and Xiumin Yan, date: 07/20/2014, view: 5883, Q&A: 0
    [Abstract] Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos.
    Motility Assay for Zebrafish Embryos
    Authors:  Michelle F. Goody and Clarissa A. Henry, date: 06/05/2013, view: 5229, Q&A: 0
    [Abstract] Analyzing the swimming ability of 2 days post fertilization zebrafish embryos can be a useful technique to study neuromuscular function. Here is a protocol for determining the time it takes for zebrafish embryos to swim a predetermined distance.
    Phalloidin Staining and Immunohistochemistry of Zebrafish Embryos
    Authors:  Michelle F. Goody and Clarissa A. Henry, date: 06/05/2013, view: 12240, Q&A: 1
    [Abstract] Fluorescent conjugated Phalloidin is a stain that allows for visualization of F-actin. In immunohistochemistry, primary antibodies and fluorescent conjugated secondary antibodies can be used to visualize subcellular localization and relative amounts of proteins of interest. Here is a protocol for Phalloidin and antibody staining of zebrafish ...