Search

Other Vertebrates

Categories

    Protocols in Current Issue
    Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis
    Authors:  Kyohei Higashi and Toshihiko Toida, date: 08/05/2017, view: 435, Q&A: 0
    [Abstract] Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (or galactose in the case of KS). Recently, a keratan sulfate (KS) disaccharide [GlcNAc6S ...
    Spinal Cord Preparation from Adult Red-eared Turtles for Electrophysiological Recordings during Motor Activity
    Authors:  Peter C Petersen and Rune W Berg , date: 07/05/2017, view: 849, Q&A: 0
    [Abstract] Although it is known that the generation of movements is performed to a large extent in neuronal circuits located in the spinal cord, the involved mechanisms are still unclear. The turtle as a model system for investigating spinal motor activity has advantages, which far exceeds those of model systems using other animals. The high resistance to ...
    Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes
    Authors:  Shujie Shi and Marcelo D. Carattino, date: 04/20/2017, view: 1042, Q&A: 0
    [Abstract] Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to ...
    Two-electrode Voltage-clamp Recordings in Xenopus laevis Oocytes:Reconstitution of Abscisic Acid Activation of SLAC1 Anion Channel via PYL9 ABA Receptor
    Authors:  Cun Wang, Jingbo Zhang and Julian I. Schroeder, date: 01/20/2017, view: 1661, Q&A: 0
    [Abstract] Two-Electrode Voltage-Clamp (TEVC) recording in Xenopus laevis oocytes provides a powerful method to investigate the functions and regulation of ion channel proteins. This approach provides a well-known tool to characterize ion channels or transporters expressed in Xenopus laevis oocytes. The plasma membrane of the oocyte is ...
    In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease
    Authors:  Sakshi Chauhan, Gajendra Kumar Azad and Raghuvir Singh Tomar, date: 01/05/2017, view: 1890, Q&A: 0
    [Abstract] Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and function. Histones are irreversibly modified by proteolytic clipping of their tail domains. The proteolytic ...
    Identification of Methylated Deoxyadenosines in Genomic DNA by dA6m DNA Immunoprecipitation
    [Abstract] dA6m DNA immunoprecipitation followed by deep sequencing (DIP-Seq) is a key tool in identifying and studying the genome-wide distribution of N6-methyldeoxyadenosine (dA6m). The precise function of this novel DNA modification remains to be fully elucidated, but it is known to be absent from transcriptional start ...
    The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
    Authors:  Piero Crespo and Berta Casar, date: 10/20/2016, view: 2935, Q&A: 0
    [Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary ...
    Chick Neural Tube Explant Culture
    Authors:  Zhanna Alekseenko, Elisabet Andersson and José M. Dias, date: 10/05/2015, view: 2867, Q&A: 0
    [Abstract] The neural tube explant culture technique allows in vitro culturing of small pieces of neural tissue isolated from e.g., chick or mouse embryonic tissue in a matrix of collagen for defined periods of time. This method can be used to study the effects of defined molecules on developmental processes such as neural progenitor ...
    Immunolocalization of Proteins in Corals: the V-type H+-ATPase Proton Pump
    Authors:  Katie L. Barott and Martin Tresguerres, date: 09/05/2015, view: 3227, Q&A: 0
    [Abstract] Here we describe the immunolocalization of a membrane-bound proton pump, the V-type H+-ATPase (VHA), in tissues and isolated cells of scleractinian corals. Immunolocalization of coral proteins requires additional steps not required for various model organisms, such as decalcification of the coral skeleton for immunohistochemistry or ...
    Electroporation of Embryonic Chick Eyes
    Authors:  Agustín Luz-Madrigal, Erika Grajales-Esquivel and Katia Del Rio-Tsonis, date: 06/20/2015, view: 3760, Q&A: 0
    [Abstract] The chick embryo has prevailed as one of the major models to study developmental biology, cell biology and regeneration. From all the anatomical features of the chick embryo, the eye is one of the most studied. In the chick embryo, the eye develops between 26 and 33 h after incubation (Stages 8-9, Hamburger and Hamilton, 1951). It originates from ...